Recombinant DMAP1 Antibody - Primary antibody, specific to DMAP1, Rabbit IgG

Cat. No.: Ab100025

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human DMAP1 Antibody, Recombinant, could be used for WB, ICC/IF and so on.

Application

• WB: 1/500-1/1000
• ICC/IF: 1/50-1/200

Protein Function

Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.

Specifications

Product Name

Recombinant DMAP1 Antibody - Primary antibody, specific to DMAP1, Rabbit IgG

Synonyms

DMAP 1 antibody | DMAP1 antibody | DMAP1_HUMAN antibody | DNA methyltransferase 1 associated protein 1 antibody | DNA methyltransferase 1-associated protein 1 antibody | DNMAP 1 antibody | DNMAP1 antibody | DNMT1 associated protein 1 antibody | DKFZp686L0

Specifications & Purity

ExactAb™, Validated, Recombinant, 0.5 mg/mL

Host species

Rabbit

Specificity

DMAP1

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within human DMAP1.

Positive Control

WB: HEK293, HeLa, THP-1, MCF7, Hep G2, Jurkat, NIH/3T3 and C6 cell lysates. ICC/IF: MCF7 cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

0.5 mg/mL

Product Line

Biological Products

Storage and Shipping

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant DMAP1 Antibody (Ab100025)

Recombinant DMAP1 Antibody (Ab100025) - Western Blot
All lanes: Recombinant DMAP1 Antibody (Ab100025) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 120.0 s

IF/ICC: Recombinant DMAP1 Antibody (Ab100025)

Recombinant DMAP1 Antibody (Ab100025) - IF/ICC
IImmunofluorescent analysis of Recombinant DMAP1 Antibody (Ab100025) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5 - 10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

Application

Dilution info

IF/ICC

1/50-1/200

WB

1/500-1/1000

Associated Targets(Human)

DMAP1 Tbio DNA methyltransferase 1-associated protein 1 (0 Activities)

Discover Target: DMAP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Application details are not specified in the Product Data for this antibody. Refer to the CoA/Spec Sheet for tested applications, recommended dilutions, and positive controls.

General protocol outlines (literature/practice-based; not item-specific):

• Western blot (WB):
  • Sample prep: Lyse cells with RIPA or nuclear extraction buffer; quantify protein.
  • Load 10–40 µg per lane; transfer to PVDF.
  • Block 5% milk/BSA in TBST; primary antibody overnight at 4°C with titration; HRP-conjugated secondary; ECL detection.
• Immunoprecipitation (IP):
  • Incubate cleared lysate with antibody 1–4 h at 4°C; capture with Protein A/G resin; wash stringently; elute with low-pH buffer or SDS sample buffer.
• ChIP:
  • Crosslink (e.g., 1% formaldehyde, 10 min), quench, sonicate chromatin to 200–500 bp; immunoprecipitate with antibody; reverse crosslinks; analyze by qPCR or sequencing.
• IF/ICC:
  • Fix with 4% PFA, permeabilize with 0.1–0.3% Triton X‑100; block with serum/BSA; incubate primary 1–2 h RT or overnight 4°C; fluorescent secondary; mount with antifade.

Critical notes:

• Include appropriate negative/positive controls (e.g., siRNA/CRISPR knockout for DMAP1 studies).
• Antibody dilution factors and incubation times must be optimized empirically for each lot and sample type.

Biological Roles

This section describes the biological context of the target protein DMAP1; it does not establish clinical utility and is provided for research background only.

General/literature overview of DMAP1:

• Function: DMAP1 (DNA methyltransferase 1-associated protein 1) is a chromatin-associated factor that interacts with DNMT1 and participates in transcriptional regulation. It has been reported to associate with histone acetyltransferase and deacetylase complexes, influencing chromatin accessibility.
• Cellular localization: Predominantly nuclear, often enriched at chromatin.
• Pathways and processes: Implicated in epigenetic regulation, DNA replication/repair interfaces, and DNA damage response signaling. DMAP1 can coordinate with TIP60/KAT5-containing complexes and may influence histone H4 acetylation states (literature).
• Model systems: Studied across human and model organisms; specific species reactivity for this antibody is not specified for this item.

Research implications:

• DMAP1 is used as a marker or node in studies of epigenetic maintenance, replication-coupled chromatin maturation, and transcriptional repression/activation contexts.

Note: The above is general biology of the target protein and not a specification or claim about this specific antibody’s performance. For validated applications and species, consult the CoA/Spec Sheet.

Buffer Applications

As an antibody reagent, this product is typically used within standard immunoassay buffers rather than as a buffering agent itself.

General buffer systems for antibody workflows (literature/practice-based; not item-specific):

• Storage/working buffers: PBS (10–50 mM phosphate, 150 mM NaCl, pH 7.2–7.4) or TBS (20–50 mM Tris, 150 mM NaCl, pH 7.4–8.0). Composition for this item is not specified; refer to CoA/Spec Sheet.
• Blocking buffers: 3–5% BSA, nonfat dry milk, or casein in TBST/PBST to reduce nonspecific binding in WB/ELISA.
• Wash buffers: PBST/TBST with 0.05–0.1% Tween‑20 commonly balances stringency and retention of complexes.
• Antigen retrieval (IHC): Citrate (pH ~6) or Tris-EDTA (pH ~9) buffers under heat can unmask epitopes on FFPE tissues; conditions require optimization for DMAP1 and are not specified for this item.

Practical notes:

• Maintain pH near neutral for stability; extreme pH can denature antibodies.
• Include protease/phosphatase inhibitors in lysis buffers for IP/WB when probing post-translationally regulated targets like DMAP1.

Item-specific buffer composition, preservatives, and recommended dilutions are not specified for this item; consult the CoA/Spec Sheet for definitive guidance.

Green Alternatives

For antibody reagents, sustainability considerations focus on cold-chain, packaging, and experimental design rather than solvent substitution.

Greener practice considerations (general guidance):

• Shipping and storage:
  • Consolidate orders to reduce shipment frequency and packaging waste. This item is shipped in an ice chest with ice pads (from Product Data); consider reuse/recycling programs for cold packs and foam.
  • Utilize -20°C storage as specified and avoid unnecessary ultra-cold storage to reduce energy consumption while maintaining stability.
• Experimental design:
  • Optimize titrations to minimize reagent consumption.
  • Use miniaturized assay formats (e.g., 96/384-well) when feasible to reduce buffer and plastic use.
• Buffer choices:
  • Prefer phosphate or Tris buffers prepared with purified water; avoid excessive single-use plastic by preparing bulk stocks.
• Alternatives and formats:
  • Where appropriate, consider recombinant fragments (Fab/scFv) or engineered binders that may offer improved expression yields and reduced resource use. Format for this item is not specified.

Trade-offs:

• Cold-chain integrity is necessary for protein stability; any attempts to reduce packaging must not compromise temperature control. Always prioritize data quality and product integrity while implementing greener lab practices.

Pharmaceutical Uses

This product is for research use only and is not intended for diagnostic, therapeutic, or clinical applications.

Contextual information (general, non-clinical):

• Antibodies as bioprocess tools: Research-grade antibodies may be used as analytical reagents in process development (e.g., Western blot reference standards) but are not suitable as GMP-grade raw materials.
• Formulation considerations (general): Stabilizers like glycerol, sucrose, trehalose, amino acids, and polysorbates are commonly used to enhance protein stability in liquid or lyophilized formats. The presence/absence of such excipients for this item is not specified; refer to CoA/Spec Sheet.
• Regulatory status: No pharmacopeial monograph or clinical-grade designation is implied by “Recombinant, ExactAb™, Validated.”

Note: Do not use this reagent in manufacturing of human or veterinary medicinal products without appropriate qualification and regulatory clearance. For any translational or diagnostic application, source a reagent with documented GMP compliance and comprehensive quality attributes.

Physical Properties

Item-specific physicochemical specifications are not provided for this antibody preparation.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer composition, and additives: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature properties for IgG-class antibodies (for context only; not item-specific):

• Approximate molecular mass: ~150 kDa for full-length IgG; fragments such as Fab (~50 kDa) or scFv (~25–30 kDa) if applicable. Exact format for this product is not specified.
• Isoelectric point (pI): Commonly in the pH 6–9 range, dependent on sequence and glycosylation.
• Solubility: High solubility in aqueous buffers (e.g., PBS, TBS). Denatured/aggregated forms can precipitate; stability improved with proper pH, ionic strength, and excipients.
• Thermal behavior: Proteins are heat-labile; prolonged exposure above refrigerated temperatures can promote denaturation and aggregation.
• UV absorbance: Typical A280 extinction coefficients vary with sequence; A280 is commonly used for concentration determination (ε depends on Trp/Tyr/Cys content). Not specified for this item.

Note: For experimental design and QC, consult the specific CoA/Spec Sheet for concentration, buffer, excipients (e.g., glycerol, BSA, preservatives), and any purification tag information if present.

Quality & Grades

Item-specific grade (from Product Data): Recombinant, ExactAb™, Validated.

• Recombinant: Indicates production via a defined expression system rather than animal immunization. Advantages include fixed sequence, lot-to-lot consistency, and reduced background from serum proteins.
• ExactAb™: Brand designation implying defined recombinant antibody provenance and tighter control over sequence and production parameters. This helps ensure reproducible performance across lots. Specific performance metrics for this SKU are not specified; refer to CoA/Spec Sheet.
• Validated: Denotes that the antibody has undergone functional testing by the manufacturer. The exact application set (e.g., WB, IF, IHC, IP, ChIP) and validation species are not specified for this item; consult the CoA/Spec Sheet for application-specific data, recommended dilutions, and validation images.

Additional quality considerations (general guidance for antibodies):

• Purity and aggregation: Analytical SEC, SDS-PAGE, and endotoxin levels are common quality attributes; not specified for this item.
• Binding specificity: Characterization typically includes ELISA, antigen competition, or orthogonal methods such as knockdown/knockout controls; details not specified for this item.
• Preservatives/stabilizers: Presence of glycerol, BSA, and/or azide may affect downstream applications (e.g., live-cell staining, crosslinking chemistry). Composition is not specified; verify on the CoA/Spec Sheet.

Reaction & Applications

Chemical reaction use is not typical for antibodies. Instead, applications center on immunodetection and enrichment of the target protein DMAP1. Manufacturer applications are not specified for this item; see CoA/Spec Sheet.

General research applications for anti-DMAP1 antibodies (literature/practice-based guidance):

• Western blot (WB): Detection of DMAP1 in whole-cell or nuclear extracts following SDS-PAGE. Employ appropriate lysis buffers and include nuclear extraction for chromatin-associated proteins.
• Immunoprecipitation (IP)/Co-IP: Enrichment of DMAP1 and associated complexes to study interactions with DNMT1 or histone-modifying enzymes; maintain nuclease and protease inhibitors.
• Chromatin immunoprecipitation (ChIP/ChIP-qPCR/ChIP-seq): To map DMAP1 occupancy at genomic loci. Requires careful crosslinking (e.g., formaldehyde), sonication optimization, and validated antibody performance.
• Immunofluorescence (IF)/Immunocytochemistry (ICC): Subcellular localization studies; DMAP1 is often nuclear. Fixation and permeabilization conditions should be optimized.

Practical tips (general):

• Use knockout/knockdown controls or peptide competition when feasible to assess specificity.
• Titrate antibody to minimize background; validate secondary antibody species compatibility.
• Keep samples and antibody cold during IP/ChIP workflows; avoid repeated freeze–thaw of the antibody to maintain affinity.

Note: The specific application validation and performance characteristics for this SKU are not specified for this item and should be confirmed from the CoA/Spec Sheet.

Reaction Conditions

No chemical reaction conditions are specified for this antibody. However, common labeling and immobilization procedures (general literature guidance) include:

• NHS-ester dye/biotin labeling:
  • Buffer: Amine-free (e.g., 50–100 mM sodium bicarbonate, pH 8.3–8.5).
  • Temperature/time: Room temperature, 30–60 min; protect from light for fluorophores.
  • Quench: Add Tris or ethanolamine; desalting to remove excess reagent.
• Maleimide-thiol coupling (after partial reduction or engineered cysteines):
  • Buffer: pH 6.5–7.0, EDTA-containing to prevent metal-catalyzed oxidation; avoid amines.
  • Temperature/time: 1–2 h at RT or 4°C overnight; maintain inert atmosphere if possible.
• Immobilization to NHS-activated resin:
  • Buffer: 0.1 M sodium bicarbonate, pH ~8.3; 1–4 h coupling at RT with gentle mixing; cap with ethanolamine.
• Enzymatic conjugation (e.g., Sortase A, glycan oxidation/oxime ligation):
  • Conditions vary by enzyme and tag; typically near-neutral pH at 4–25°C.

Expected outcomes and controls:

• Degree of labeling: Often targeted at 2–6 labels per IgG; verify by UV/Vis for dyes or HABA/Avidin for biotin.
• Functional testing: Post-conjugation, re-validate binding by ELISA or WB.

Note: These are general conditions; buffer composition and stability characteristics for this SKU are not specified and should be verified on the CoA/Spec Sheet before modification.

Safety & Handling

GHS/SDS information for this specific item is not provided in the Product Data. Antibodies are generally considered low hazard in typical laboratory use, but always handle as biological materials with appropriate precautions.

• GHS classification, signal word, H-statements, and pictograms: Not specified for this item; refer to the SDS.
• General laboratory PPE: Lab coat, gloves (nitrile or equivalent), and safety glasses. Change gloves regularly and after contamination.
• Handling guidance:
  • Work on clean, dedicated surfaces to avoid cross-contamination.
  • Keep cold during use (ice bath or cold block) to minimize thermal stress.
  • Avoid repeated freeze–thaw cycles, which can cause aggregation and loss of activity.
• Incompatibilities (general):
  • Avoid strong acids/bases and oxidizing agents that can denature proteins.
  • Avoid surfactant-free vigorous mixing and foaming; shear can denature antibodies.
• First aid (general overview; defer to SDS):
  • Skin/eye contact: Rinse with water. Remove contaminated clothing. Seek medical attention if irritation persists.
  • Ingestion/inhalation: Unlikely in standard use; seek medical advice if exposure occurs.
• Spill and disposal:
  • Small spills: Absorb with disposable towels, clean with suitable detergent/disinfectant.
  • Disposal: Follow institutional biosafety procedures and local regulations for proteinaceous lab waste.
• Biosafety note: This material is for research use only; not for human or animal diagnostic or therapeutic use.

Solvent Selection

This product is an aqueous protein reagent rather than a small-molecule solute; traditional organic solvent selection is not typically applicable.

Practical considerations for antibody handling (general guidance):

• Recommended medium: Physiological aqueous buffers such as PBS (pH ~7.2–7.4) or TBS (pH ~7.4–8.0) with optional stabilizers (e.g., 10–50% glycerol) to improve freeze–thaw stability. Buffer composition for this item is not specified; refer to CoA/Spec Sheet.
• Avoid: Strong organic solvents (acetone, acetonitrile, DMSO at high %), extremes of pH, and high ionic detergents unless specifically required by the protocol.
• Detergents: Low concentrations of nonionic surfactants (e.g., 0.01% Tween-20) can reduce nonspecific binding in immunoassays; compatibility depends on the application and is not item-specific.

When to choose this antibody versus alternatives:

• Select a DMAP1 antibody when probing chromatin regulation, DNMT1-interacting complexes, or DNA damage response pathways. If performing denaturing vs native assays, consider format (full IgG vs fragments) and species reactivity. These attributes for this item are not specified; verify with CoA/Spec Sheet.

Storage & Reconstitution

Item-specific storage from Product Data:

• Store at -20°C.
• Avoid repeated freezing and thawing.
• Shipped in an ice chest with ice pads.

General handling and reconstitution guidance (not item-specific; follow CoA/Spec Sheet if provided):

• Upon receipt: Inspect for container integrity and any signs of thawing. Centrifuge briefly to collect contents at the bottom of the vial before first use.
• Aliquoting: Immediately prepare small, single-use aliquots under sterile conditions to prevent multiple freeze–thaw cycles.
• Working buffer: If buffer composition is not optimal for your application, gentle buffer exchange (spin desalting columns) into PBS or TBS at neutral pH is common. Composition for this item is not specified; verify compatibility before exchange.
• Stabilizers: For long-term storage at -20°C, many labs add sterile glycerol to 40–50% v/v to reduce freeze–thaw damage; only adjust if compatible with your application and after confirming current formulation on the CoA/Spec Sheet.
• Temperature excursions: Minimize time at room temperature; keep on ice while setting up experiments.
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet. Discard if turbidity, precipitate, or loss of performance is observed.

Note: This product is for research use only. Follow institutional biosafety procedures when handling and disposing of antibody solutions.

Structure & Identity

This product is a recombinant research antibody directed against DMAP1 (DNA methyltransferase 1-associated protein 1).

• Item-specific identifiers:
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES/InChI/InChIKey/CAS/CID: Not applicable for protein biologics; not specified for this item.
• Grade/Purity (from Product Data):
  • Recombinant, ExactAb™, Validated.
• Structural features (general description of antibodies; literature):
  • Typical recombinant IgG antibodies are Y‑shaped glycoproteins comprised of two identical heavy chains (~50 kDa each) and two identical light chains (~25 kDa each) linked by disulfide bonds, forming variable (Fab) regions for antigen binding and an Fc region for effector functions.
  • Variable domains contain complementarity-determining regions (CDRs) that define antigen specificity. Recombinant production confers a defined amino acid sequence and batch-to-batch consistency.
• Target overview (general knowledge):
  • DMAP1 is a chromatin-associated factor originally identified as a DNMT1-interacting protein, implicated in transcriptional regulation, histone acetylation/deacetylation complexes, and DNA damage responses.

2D/primary structure description (general):

• Antibodies do not have a single small-molecule 2D structure; the primary structure is a polypeptide sequence with immunoglobulin domains forming beta-sandwich folds. Epitope and clone identifiers for this specific reagent are not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

While antibodies are not classical synthetic building blocks, they can be chemically modified for research tools.

General/literature utility of antibodies in chemical biology:

• Conjugation chemistry: Primary amines (Lys) enable NHS-ester coupling to fluorophores, biotin, or haptens; reduced interchain disulfides (hinge region) allow maleimide-thiol coupling for site-directed labeling. Enzymatic methods (e.g., Sortase A, glycan remodeling) provide more controlled conjugation.
• Affinity matrices: Covalent immobilization on activated agarose (e.g., NHS-activated Sepharose) creates specific IP columns to purify DMAP1-containing complexes.
• Proximity labeling and crosslinking: Photoaffinity or chemical crosslinkers (e.g., DSS, BS3) can stabilize protein complexes prior to MS analysis; ensure compatibility with antibody stability and downstream detection.
• Antibody fragments: Enzymatic digestion (papain, pepsin) generates Fab/F(ab')2 fragments with distinct properties (smaller size, improved tissue penetration); format for this item is not specified.

Caveats:

• Over-labeling can impair antigen binding; degree of labeling (DOL) should be quantified and kept within recommended ranges.
• Preservatives (e.g., azide) can interfere with certain conjugations or enzyme-mediated steps; confirm buffer composition for this item on the CoA/Spec Sheet before modification.

Target Specificity

Only information from Product Data is reported in this section.

• Antigen/Target: DMAP1 (DNA methyltransferase 1-associated protein 1).
• Clone, isotype, host species, species reactivity, epitope, and cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Validation status: “Validated” (from Product Data). Specific validated applications and species are not provided here; consult CoA/Spec Sheet.

Note: For definitive specificity, review manufacturer validation data, including knockout/knockdown controls, peptide competition, or orthogonal assays as applicable.

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant DMAP1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.