TIP49A Antibody - Primary antibody, specific to RUVBL1, Rabbit IgG

Cat. No.: Ab131386

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.14 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human TIP49A Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.

Specifications

Product Name

TIP49A Antibody - Primary antibody, specific to RUVBL1, Rabbit IgG

Synonyms

49 kDa TATA box binding protein interacting protein antibody | 49 kDa TATA box-binding protein-interacting protein antibody | 49 kDa TBP interacting proteinantibody | 49 kDa TBP-interacting protein antibody | 54 kDa erythrocyte cytosolic protein antibody

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.14 mg/mL

Host species

Rabbit

Specificity

RUVBL1

Immunogen

Fusion protein of human RUVBL1 (AA 57-345).

Positive Control

WB: Raji, K562, HT-29, A549, NIH/3T3 and C6 cell lysates. IHC: Human ovarian cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

1.14 mg/mL

Product Line

Biological Products

Storage and Shipping

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: TIP49A Antibody (Ab131386)

TIP49A Antibody (Ab131386) - Western Blot
All lanes: TIP49A Antibody (Ab131386) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 15.1 s

IHC: TIP49A Antibody (Ab131386)

TIP49A Antibody (Ab131386) - IHC
Immunohistochemistry analysis of paraffin-embedded Human ovarian cancer using TIP49A Antibody (Ab131386). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: TIP49A Antibody (Ab131386)

TIP49A Antibody (Ab131386) - IF/ICC

IF analysis of TIP49A Antibody (Ab131386) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with TIP49A Antibody (Ab131386) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

IHC

1/50 - 1/100

IF/ICC

1/200

WB

1/500-1/1000

Associated Targets(Human)

RUVBL1 Tbio RuvB-like 1 (0 Activities)

Discover Target: RUVBL1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TIP49A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.