AMF Mouse mAb - Anticuerpo primario, specific to AMF, IgG1

Cat. No.: Ab088587

CALIDAD & PUREZA Carrier Free, ExactAb™, Validated, 1.0mg/mL

Application

ELISA, IF/ICC, IHC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

1B7D7

Conjugation

Unconjugated

Descripción general

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

Especificaciones

Product Name

AMF Mouse mAb - Anticuerpo primario, specific to AMF, IgG1

Sinónimos

AMF antibody|Aurocrine motility factor antibody|Autocrine motility factor antibody|DKFZp686C13233 antibody|EC 5.3.1.9 antibody|Glucose phosphate isomerase antibody|Glucose-6-phosphate isomerase antibody|GNPI antibody|GPI antibody|Gpi1 antibody|Hexose monophosphate isomerase antibody|Hexosephosphate isomerase antibody|Neuroleukin antibody|NLK antibody|Oxoisomerase antibody|PGI antibody|PHI antibody|Phosphoglucose isomerase antibody|Phosphohexomutase antibody|Phosphohexose isomerase antibody|Phosphosaccharomutase antibody|SA 36 antibody|SA-36 antibody|SA36 antibody|Sperm antigen 36 antibody|AMF|Aurocrine motility factor|Autocrine motility factor|Glucose phosphate isomerase|Glucose-6-phosphate isomerase|GNPI|GPI|Gpi1|Hexose monophosphate isomerase|Hexosephosphate isomerase|Neuroleukin|NLK|Oxoisomerase|PGI|PHI|Phosphoglucose isomerase|Phosphohexomutase|Phosphohexose isomerase|Phosphosaccharomutase|SA 36|SA-36|SA36|Sperm antigen 36

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Especies hospedadoras

Ratón

Especificidad

AMF

Inmunógeno

Purified recombinant fragment of human GPI(AA:1-558) expressed in E.Coli.

Control positivo

WB: Hep G2, A431, K562, HeLa, A549, MCF-7 cell lysates. ICC/IF: L-02 cells. IHC: Human Kidney tissues.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150kDa

Método de purificación

Protein G purified

Concentración

1.0mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Imágenes

Western Blot: AMF Mouse mAb (Ab088587)

AMF Mouse mAb (Ab088587) - Western Blot
All lanes: AMF Mouse mAb (Ab088587) at 1/1000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 63 kDa
Observed band size: 57 kDa

ICC/IF: AMF Mouse mAb (Ab088587)

AMF Mouse mAb (Ab088587) - ICC/IF
AMF Mouse mAb (Ab088587) at 1/1000 dilution staining AMF in L-02 cells by Immunocytochemistry/ Immunofluorescence. An AF488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. Green staining in image show positive staining with AMF Mouse mAb, actin filaments were stained red with DY-554 phalloidin and nuclei stained blue with DRAQ5 fluorescent DNA dye.

IHC: AMF Mouse mAb (Ab088587)

AMF Mouse mAb (Ab088587) - IHC
Immunohistochemical analysis of paraffin-embedded human Kidney tissues using AMF Mouse mAb (Ab088587) .

Aplicación

Aplicación

Dilution info

WB

1/500-1/2000

IF/ICC

1/200-1/1000

ELISA

1/10000

IHC

1/200-1/1000

Objetivos asociados (humanos)

GPI Tbio Glucose-6-phosphate isomerase (0 Activities)

Discover Target: GPI

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción