Secondary antibody

Step-by-step guidelines for using Rat IgG2b Mouse mAb (HRP) Ab349244 in ELISA, WB, and IHC workflows for optimal detection results.

Step-by-step protocol for Rat IgG2c Mouse mAb (HRP) Ab350678: optimal dilutions, storage, and detection in ELISA and Western blot.

When choosing a secondary antibody for western blotting, one of the main selection criteria is the species of the primary antibody that the secondary antibody binds to.

In immunology experiments, it is often necessary to select different secondary antibodies to meet the experimental requirements. Usually, there may be several types of secondary antibodies available for a particular experiment, and the following aspects need to be considered to select the most ...

Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.

How should I choose the secondary antibody?

Apoptosis is a tightly regulated cell death mechanism, the core of which is the activation of caspases. Immunofluorescence staining is an effective technique that can simultaneously detect caspases and other apoptosis-related proteins in cell samples.

IgG, the key factor of Humoral immunity response, is the main immunoglobulin in blood, lymph, cerebrospinal fluid and peritoneal fluid. IgG accounts for approximately 75% of total serum immunoglobulins and is the only immunoglobulin that can penetrate the human placenta. It is primarily ...