Avidin from egg white, CAS No.1405-69-2

CAS: 1405-69-2 Cat. No.: A103033 Número EC: 215-783-6

CALIDAD & PUREZA Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Accession #

P02701

Bioactivity

≥10 U/mg solid; >10 U/mg protein

Descripción general

Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.

Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.

Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in diagnostic kit development and manufacturing.

Application：

Avidin from egg white can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Especificaciones

Product Name

Avidin from egg white, CAS No.1405-69-2

Sinónimos

Avidin from egg white | Avidin, chiken egg white | Avidin, Egg White | Anti-biotin protein | Immunopure Avidin | AVD

Grado

Bioactive, Native

Especificaciones y pureza

Bioactive, Native, ≥98%(SDS-PAGE), ≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Mecanismos bioquímicos y fisiológicos

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). Post-translational: N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)

Bioactividad

≥10 U/mg solid; >10 U/mg protein

Número de adhesión

P02701

Peso molecular previsto

64,000 (tetramer of 16,000 MW subunits)

CAS

1405-69-2

Tipo de molécula

Proteína

Product Line

Productos biológicos

Almacenamiento y envío

Concentración

≥10 U/mg solid; >10 U/mg protein; ≥0.9 mg protein/mg solid (see COA for assay result)

Reconstitución

Reconstitute in 0.1 M phosphate buffer, pH 6.8.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (36 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definición de unidad

One unit will bind one microgram of d-biotin.