Alfalfa stem cell wall protein extraction experiment
The plant cell wall is a highly dynamic and chemically active component of the plant cell. The main components of the cell wall are polysaccharides, as well as
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3228 articles
Extraction and solubilization experiments of plant plasma membrane proteins
In all living cells the plasma membrane (PM ) exists as an interface between the cytoplasm and the environment and is one of the most complex and differentiated
Experiments on the solubilization of proteins by descaling and leaving agents prior to bidirectional electrophoresis
Because of its outstanding ability to separate total proteins, bidirectional gel electrophoresis can be applied to the separation of all types of proteins. Howe
Plant proteomics and glycosylation experiments
Like other eukaryotic cells, glycosylation is one of the most studied post-translational events in plant cells. Depending on the type of connection between the
Peptide mass fingerprinting and protein identification by MALDI-TOF method
MALDI-TOF peptide mass fingerprinting (PMF) is a faster and more convenient method for identifying proteins. The genomes studied have been sequenced and annotat
Protein Edman sequencing experiments on 2D gels
Immunoblotting using polyvinylidene difluoride (PVDF ) membranes is the most commonly used method for Edman sequencing. Protein samples are transferred from a 2
2-DE PROTICdb database experiment for proteomics
PROTICdb is a web-based database designed primarily to store and analyze plant proteomic data obtained by 2D polyacrylamide electrophoresis and mass spectrometr
Protein identification using nanoscale liquid chromatography-tandem mass spectrometry
Tandem mass spectrometry is an effective technique for peptide characterization based on the fragmentation pattern of the peptide, also known as MS/MS scanning.
Experiments in multivariate analysis of proteomic data
We provide background on multivariate analysis of proteomics data and also provide methods for data transformation of different data packages. This technique ca