Principles, materials and procedures for denaturing agarose gel electrophoresis of RNA
This experiment is mainly used to determine the integrity of RNA extracts.
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Whole blood RNA extraction
Whole blood RNA extraction can be used (1) to study the mechanism of RNA interference, etc.; (2) as a template for reverse transcription; and (3) for other stud
Construction of siRNA expression vectors
The construction of siRNA expression vectors can be applied to: (1) as a powerful tool for gene function analysis in the post-genomic era; (2) genomics and cell
Northern blotting and narrow line hybridization analysis of RNA
Northern blot is one of the most rigorous methods to study gene expression, which can quantitatively analyze the expression abundance of a specific mRNA in tiss
Isolation of cytoplasmic RNA from mammalian cells
The following method is a simple and effective method for purifying cytoplasmic RNA from tissue culture cells, which appeared at an earlier stage of RNA isolati
cDNA synthesis technology
After the mRNA of the target gene has been identified by in vitro cell-free translation, the first strand of cDNA can be synthesized in the steps described in t
Total RNA extraction from egg and embryo cells
This method is a simple and effective way to isolate RNA from oocytes, fertilized eggs and fetal cells of frogs, sea urchins, periphyton, worms and flies.
Effect of different methods of treating tissues on the yield of extracted RNA
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
Extraction and isolation of rat liver rRNAs
Rat liver was homogenized, the supernatant was centrifuged, and RNA was extracted with a mixture of phenol and m-cresol. rRNA and mRNA were separated in the pre