Ligation of RNA molecules with T4 RNA ligase
T4RNA ligases have been used to generate many site-specific modified RNAs, especially oligonucleotide-modified and tRNA-modified with antisense codon tRNAs.In a
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T4 DNA ligase ligates RNA molecules
T4 DNA Ligase joins double-stranded complexes, including RNA-DNA heteroduplexes and RNA-RNA heteroduplexes, defectively.
RNA radiolabeling
Intramolecular labeling of RNA during transcription, post-transcriptional labeling of the 5' end of RNA using T4 polynucleotide kinase and labeling of the 3' en
nuclear transcription analysis
Nuclear runoff (runoff) analysis can be used to examine the transcriptional efficiency of specific genes in isolated cell nuclei. For runoff analysis, the nucle
Deoxyribonuclease cleaves RNA at a specific site
Deoxyribonucleases are oligodeoxyribonucleotides consisting of about three dozen deoxyribonucleotides, which are easy to prepare and convenient to use, and are
In vitro translation system for prokaryotic cells
The S30 family of extracts is divided into three categories: the E. coli S30 Extract System for in vitro translation of circular DNA, the E. coli S30 Extract Sy
Translation of mRNA in wheat germ extracts
Wheat germ extract in vitro translation system for efficient translation of mRNA in viruses and eukaryotic cells in a heterogeneous cell-free system
An in vitro translation system for African Xenopus oocytes
The African Xenopus oocyte in vitro translation system is capable of sustained modification and processing of most exogenous mRNAs due to its ability to translo
mRNA 3' end processing in vitro
There are two strategies for in vitro experiments; one is to co-incubate a plasmid containing the target gene at the Po ly( A) locus with cell-free extracts to