Extraction and isolation of rat liver rRNAs
Rat liver was homogenized, the supernatant was centrifuged, and RNA was extracted with a mixture of phenol and m-cresol. rRNA and mRNA were separated in the pre
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RNA extraction from fresh and frozen blood
Whole blood contains nucleated leukocytes, so it is easy to extract RNA from blood, but red blood cells are rich in ribonuclease, so it is difficult to isolate
Extraction of RNA from tissues by the CsCl method
Because some tissues (e.g., pancreas and uterus) contain high concentrations of endogenous RNAases, more care must be taken in purifying tissue-derived RNA.
Effect of different methods of treating tissues on the yield of extracted RNA
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
Synthesize 5' capped transcripts
Some RNA transcripts require an m7 G(5')Ppp(5')G cap at the 5' end, and they have higher translational efficiency only in cell-free extracts or in African Xenop
RNA extraction from formalin-fixed, paraffin-embedded tissues
Formalin-fixed, paraffin-embedded tissues from the pathology department can be used for RNA expression analysis. RNA isolated from biopsy, surgical, and cadaver
nuclear transcription analysis
Nuclear runoff (runoff) analysis can be used to examine the transcriptional efficiency of specific genes in isolated cell nuclei. For runoff analysis, the nucle
Deoxyribonuclease cleaves RNA at a specific site
Deoxyribonucleases are oligodeoxyribonucleotides consisting of about three dozen deoxyribonucleotides, which are easy to prepare and convenient to use, and are
T4 DNA ligase ligates RNA molecules
T4 DNA Ligase joins double-stranded complexes, including RNA-DNA heteroduplexes and RNA-RNA heteroduplexes, defectively.