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Laboratory Protocols & SOPs Library

3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.

3228 articles
Laboratory Protocols & SOPs Library

IL-10 biological activity assay

In the presence of mouse IL-4 (Mil-4), IL-10 stimulated proliferation of the D36 mouse mast cell line. In contrast, single mIL4 or hIL-10 had only very low prol

2025-06-10 03:34:22 Updated
Laboratory Protocols & SOPs Library

IL-1 biological activity assay

Interleukin-1 (IL-1) is a cytokine mainly synthesized and secreted by activated monocyte-macrophage cells, and has various biological functions such as activati

2025-06-10 03:34:22 Updated
Laboratory Protocols & SOPs Library

IL-2 biological activity assay

Interleukin-2 (IL-2) is a cell proliferation factor secreted by activated helper T cells, which has the effect of promoting T cell proliferation and maintaining

2025-06-10 03:34:22 Updated
Laboratory Protocols & SOPs Library

Interleukin 8 (IL- 8) Assay

Il-8 is a polypeptide with a molecular weight of 8-10 kD, which has chemotactic effects on neutrophils, T lymphocytes, and alkaliphilic granulocytes, and activa

2025-06-10 03:34:22 Updated
Laboratory Protocols & SOPs Library

IL-8 Biological Activity Assay

There are activating and chemotactic effects on neutrophils in IL-8, and the biological activity of IL-8, i.e., chemotactic activity on neutrophils, can be dete

2025-06-10 03:34:22 Updated
Laboratory Protocols & SOPs Library

IL-4 biological activity assay

IL-4 is mainly a cytokine produced by the activation of TH cells.CT.4S cells, an IL-4-dependent cell line, show hyporesponsiveness to IL-2, and their proliferat

2025-06-10 03:34:22 Updated
Laboratory Protocols & SOPs Library

IL-6 biological activity assay

MH60.BSF2 is an IL-6-dependent cell line, and the amount of MH60.BSF2 cell proliferation was positively compared with the biological activity of IL-6.

2025-06-10 03:34:22 Updated
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