Analysis of mitogen-activated protein kinase (MAPK) activity in T cells
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Analysis of inositol phospholipid turnover during lymphocyte activation
Receptor-mediated activation of phospholipase C (P L C) induces hydrolysis of membrane inositol phospholipids to produce diacylglycerol (D A G) and water-solubl
Blotting assay for anti-phosphorylated tyrosine
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Detection of tyrosine protein kinase by immunocomplex methods
The low abundance of tyrosine protein kinase (T P K ) expression and its rapid activation and inactivation make it difficult to purify using conventional bioche
Preparation of mouse T-cell hybrid tumors
T-cell hybridomas can be obtained by fusing activated T cells with tumor cells. Heterogeneous hybridomas can be cloned by limited dilution to obtain hybridomas
ELISPOT method for detection of cytokine-secreting cells
Enzyme-linked immunospot test (ELISPOT) is based on the principle that cells that secrete certain protein molecules (e.g. cytokines) are able to secrete a relat
IL-2 and IL-4 assays
A method is described for the detection and quantification of one of the cytokines in the presence of the other using neutralizing antibodies to murine IL-2 and
Detection of IL-15
The ELISA method can be used to detect human IL-15 in culture supernatants and serum samples. This method can also be used for the detection of monkey IL-15, bu
Detection of IL-13
ELISA is highly specific for human or mouse IL-13 and does not recognize other cytokines in the sample. Author: J.E. Colligan et al, Translator: Xuitao Cao et a