3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Immunoprecipitation of natural proteins is a classic method for studying natural protein interactions, which relies on the specific binding between antibodies and proteins (antigens). This technique can be used to qualitatively detect proteins, determine the physiological role of proteins in ...
Phosphoprotein western blot is a widely used technique for detecting and analyzing the phosphorylation status of proteins, which is essential for understanding various biological processes and signal transduction pathways. Some common application scenarios include signal transduction research, ...
Amine-reactive probe labeling techniques are widely used for the labeling and tracking of biomolecules, especially in the modification of proteins and nucleic acids.
The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is an electrophoretic technique used to separate and analyze large protein complexes. It preserves protein complexes in their native state under non-denaturing conditions by treating samples with mild detergents and negatively charged dyes ...
Plasmid transformation is a commonly used technique in molecular biology that introduces foreign plasmid DNA into host cells such as E. coli, enabling gene cloning, gene expression, and other genetic manipulations. Competent E. coli cells are made more permeable to plasmid DNA through physical ...
For western blotting, incubate the membrane with primary antibody diluted in 1×TBST (0.1% Tween®20) containing 5% w/v BSA or nonfat dry milk overnight at 4°C on a shaker. Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and antibody dilution.
This method provides a recipe of lysate and buffer preparation for lysing cells or tissues for western blot analysis.
Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...