Experimental environmental metabolomics studies with 1H-NMR spectroscopy
Environmental metabolomics is a branch of metabolomics that focuses on the metabolic changes of organisms in response to environmental stress. Because this appr
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Preparation of pH standard color scale
Most cell lines grow best at pH 7.4, with little variation between cell lines; however, some normal fibroblast cell lines grow best at pH 7.4 to 7.7. However, s
Hidradenitis elegans nematode RNA preparation
A modification of the standard method for RNA preparation from mammalian tissues and Drosophila that focuses on rapid lysis of Juniperus communis and homogeneou
Embryonic stem cell in vitro culture and directed induction of differentiation experiments
Feeder cells are necessary for embryonic-derived stem cells including embryonic cancer cells (EC), embryonic stem cells (ES), and primordial germ cells (EG) to
HIV-2 Vectors for Human Gene Therapy: Design, Construction, and Therapeutic Prospects
Lentiviral vectors can be designed as replication-incomplete or replication-condition-dependent (motile) vectors. HIV-2 vectors are more effective in gene thera
Stem Cell and Gene Therapy
Gene therapy refers to the suppression, replacement or supplementation of defective genes at the genetic level through the regulation of the expression of targe
SIV Vectors as DNA Delivery Mediators
Provides some methods on how to use the miniature SIV packaging system and SIV gene transfer vectors to obtain high titer and safe lentiviral vectors developed
Stem Cell Preservation and Resuscitation
Hematopoietic stem cell transplantation is an effective treatment for malignant hematological disorders, oncological radiotherapy and severe radiation sickness
Construction of multiple cis-trans vectors linked by 2A polypeptides
This chapter describes methods for expressing multiple proteins from a single readable frame by using 2A polypeptide-linked polycis-trans vectors. When these sm