GSDMD Antibody - Anticuerpo primario, specific to GSDMD, Rabbit IgG

Cat. No.: Ab106592

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Application

IF/ICC, WB

Host species

Conejo

Reactive species

Humano, Ratón

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human GSDMD Antibody, Polyclonal, could be used for WB and so on.

Specifications

Product Name

GSDMD Antibody - Anticuerpo primario, specific to GSDMD, Rabbit IgG

Sinónimos

Gasdermin domain-containing protein 1 | 1810036L03Rik antibody | DF 5L antibody | DF5L antibody | DFNA 5L antibody | DFNA5L antibody | FKSG 10 antibody | FKSG10 antibody | FLJ12150 antibody | Gasdermin antibody | Gasdermin D antibody | Gasdermin domain co

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Especies hospedadoras

Conejo

Especificidad

GSDMD

Inmunógeno

Recombinant GSDMD expressed in E.coli (AA 232-484)

Control positivo

WB: A431, U2OS and NIH/3T3 cell lysates. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Antigen affinity purified and Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: GSDMD Antibody (Ab106592)

GSDMD Antibody (Ab106592) - Western Blot
All lanes: GSDMD Antibody (Ab106592) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 32, 45, 51kDa
Exposure time: 30.0 s

IF/ICC: GSDMD Antibody (Ab106592)

GSDMD Antibody (Ab106592) - IF/ICC
IF analysis of GSDMD Antibody (Ab106592) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GSDMD Antibody (Ab106592) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicación

Aplicación

Dilution info

WB

0.01-2 µg/mL

IF/ICC

2.5 µg/mL

Objetivos asociados (humanos)

GSDMD Tchem Gasdermin-D (0 Activities)

Discover Target: GSDMD

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GSDMD Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.