SA-HRP

Cat. No.: np156148

GRADE & PURITY Azide Free, High Performance, Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed.

Descripción general

Background

Streptavidin is a non-glycosylated protein from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.

Recommended Usage：

WB: 1:10000 -1:100000

ELISA: 1:1000-1:10000

Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Specifications

Product Name

SA-HRP

Sinónimos

SA-HRP (HRP-labeled Streptavidin) | Streptavidin-Horseradish Peroxidase | SAv-HRP | SA V1 | SA V2 | streptavidin | Streptavidin V1 | Streptavidin V2

Grado

Azide Free, High Performance

Especificaciones y pureza

Azide Free, High Performance, Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed.

Especie

Streptomyces avidinii

Secuencia

MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLG STFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWT VAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVG HDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ

Longitud de la proteína

Full length protein

Conjugación

HRP

Fuente

Native

Nota

This product can replace S292620.

Tipo de molécula

Proteína

Almacenamiento y envío

Forma

Liquid

Concentración

Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed.

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle and protected from prolonged exposure to light. Prepare working dilution on day of use.

Imágenes

WB-Streptavidin (HRP) (np156148)

WB-Streptavidin (HRP) (np156148)
Various whole cell lysates were subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:10000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 10s.

ELISA-Streptavidin (HRP) (np156148)

ELISA-Streptavidin (HRP) (np156148)
ELISA results of Streptavidin (HRP) (np156148) tested against Biotin-BSA. Each well was coated in duplicate with 1 ng of Biotin-BSA.

WB-Streptavidin (HRP) (np156148)

WB-Streptavidin (HRP) (np156148)
HT-29 whole cell lysate was subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:5000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 8s.

Aplicación

• Aplicación: Dilution info
• WB:

  1:10000-1:100000;

• ELISA:

  1/1000-1/10000 ;