TBP Mouse mAb - Anticuerpo primario, specific to TBP, Mouse IgG2a

Cat. No.: Ab130268

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, See COA

Application

WB

Host species

Ratón

Reactive species

Cabra, Cerdo, Conejillo de Indias, consejo, Humano, Oveja, Ratón

Isotype

Mouse IgG2a

Clone Number

C3

Conjugation

Unconjugated

Descripción general

Mouse anti Human TBP Antibody, Monoclonal (C3), could be used for WB and so on.
Application:
WB: 0.5-2µg/mL
Protein Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Especificaciones

Product Name

TBP Mouse mAb - Anticuerpo primario, specific to TBP, Mouse IgG2a

Sinónimos

GTF2D antibody|GTF2D1 antibody|HDL4 antibody|MGC117320 antibody|MGC126054 antibody|MGC126055 antibody|SCA17 antibody|TATA binding factor antibody|TATA box factor antibody|TATA sequence binding protein antibody|TATA sequence-binding protein antibody|TATA-binding factor antibody|TATA-box binding protein N-terminal domain antibody|TATA-box factor antibody|TATA-box-binding protein antibody|TBP antibody|TFIID antibody|Transcription initiation factor TFIID TBP subunit antibody|TBP|GTF2D|GTF2D1|HDL4|SCA171|TFIID|Anti-TBP antibody|TATA sequence-binding protein|TATA-binding factor|TATA-box factor|Transcription initiation factor TFIID TBP subunit

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Especies hospedadoras

Ratón

Especificidad

TBP

Inmunógeno

Recombinant TBP expressed in E.coli (AA 25-339).

Control positivo

WB: Jurkat, HEK293, HeLa, K562, C6, NIH/3T3 cell lysates.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A+G Affinity Chromatography

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: TBP Mouse mAb (Ab130268)

TBP Mouse mAb (Ab130268) - Western Blot
All lanes: TBP Mouse mAb (Ab130268) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 38 kDa
Observed band size: 43&36 kDa
Exposure time: 5.1 s

Aplicación

Aplicación

Dilution info

WB

0.5-2 µg/mL

Objetivos asociados (humanos)

TBP Tbio TATA-box-binding protein (0 Activities)

Discover Target: TBP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TBP Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.