Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Application
WB: 1/2,000-1/10,000 ; Predicted molecular weight: 54 kDa
Flow: 0.008 μg/mL – 1 μg/mL
ICC/IF: 2 μg/mL
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 25.0 s
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.5 kDa
Exposure time: 25.0 s
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 10.1 s
Vimentin Mouse mAb (Ab179444) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab179444) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Vimentin Mouse mAb (Ab179444) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab179444) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC | 2 μg/mL |
| Flow Cytometry |
0.008-1μg/mL
|
| WB | 1/2,000-1/10,000 ; Predicted molecular weight: 54 kDa |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24R0400481 | Certificate of Analysis | Apr 26, 2024 | Ab179444 |
| ZJ24F0102679 | Certificate of Analysis | Feb 01, 2024 | Ab179444 |
| ZJ24F0102678 | Certificate of Analysis | Feb 01, 2024 | Ab179444 |
| ZJ24F0102677 | Certificate of Analysis | Feb 01, 2024 | Ab179444 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →
