Vimentin Mouse mAb - Anticuerpo primario, specific to Vimentin, IgG2a

Cat. No.: Ab179919

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, See COA

Application

Flow Cytometry, WB

Host species

Ratón

Reactive species

consejo, Humano, Ratón

Isotype

Mouse IgG2a

Clone Number

AB08/4A9

Conjugation

Unconjugated

Descripción general

Application
WB: 1/2,000-1/100,000 ; Predicted molecular weight: 54 kDa
Flow: 0.016 μg/mL - 0.5 μg/mL

Specifications

Product Name

Vimentin Mouse mAb - Anticuerpo primario, specific to Vimentin, IgG2a

Sinónimos

CTRCT30 antibody | Epididymis luminal protein 113 antibody | FLJ36605 antibody | HEL113 antibody | VIM antibody | VIME_HUMAN antibody | Vimentin antibody | Anti-Vimentin Antibody | Vimentin Monoclonal Antibody | Anti-Human VIM/Vimentin Antibody | Mouse an

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA

Especies hospedadoras

Ratón

Especificidad

Vimentin

Inmunógeno

Recombinant full length protein corresponding to human Vimentin

Control positivo

WB: HeLa, HEK293, Jurkat, K562, A549, NIH/3T3, C6 and Raji whole cell lysates Flow: HeLa cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotipo

IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C for short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Vimentin Mouse mAb (Ab179919)

Vimentin Mouse mAb (Ab179919) - Western Blot
All lanes: Vimentin Antibody (Ab179919) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 53.7 kDa
Observed band size: 54.9 kDa
Exposure time: 20.0 s 

Flow Cytometry: Vimentin Mouse mAb (Ab179919)

Vimentin Mouse mAb (Ab179919) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab179919) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.

Aplicación

• Aplicación: Dilution info
• WB:

  1/2,000-1/100,000 ; Predicted molecular weight: 54 kDa ;

• Flow Cytometry:

  0.016 μg/mL - 0.5 μg/mL ;

Objetivos asociados (humanos)

VIM Tbio Vimentin (0 Activities)

Discover Target: VIM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Vimentin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.