2X Laemmli Sample Buffer - 2X , CAS No.X378399

CAS: X378399 Cat. No.: X378399

GRADE & PURITY 2X

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.
2X Laemmli Sample Bufferis for SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer补充资料This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.

Specifications

Specifications & Purity

2X

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• WGK Germany: 3
• UN Number: 3082
• Packing Group: III

Chemical and Physical Properties

• Solubility: Soluble in water.pH'~6.8