5-DBCO-PEG4-dUTP - ≥95%, 10mM solution in 100 mM Tris-HCl , CAS No.D487788

CAS: D487788 Cat. No.: D487788 Molecular Weight: 1057.83 (free acid)

GRADE & PURITY ≥95%, 10mM solution in 100 mM Tris-HCl

Synonyms

5-DBCO-PEG₄-dUTP

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice
to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins

Applications:
Incorporation into DNA/cDNA by
PCR with Taq polymerase in-house data

pH: 7.5 ±0.5
Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Specifications

Synonyms

5-DBCO-PEG₄-dUTP

Specifications & Purity

≥95%, 10mM solution in 100 mM Tris-HCl

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥95%

Names and Identifiers

• Molecular Weight: 1057.83 (free acid)

Chemical and Physical Properties

• Solubility: Tris buffer: 100mM (pH 7.5)