This compound belongs to the class of organic compounds known as peptoid-peptide hybrids. These are compounds containing a peptoid-peptide backbone, which consists alternating amino acid and n-substituted amino acids linked to each other by a peptide bond.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Propriétés chimiques et physiques
Poids moléculaire
994.200 g/mol
XLogP3
0.000
Hydrogen Bond Donor Count
11
Hydrogen Bond Acceptor Count
12
Rotatable Bond Count
30
Exact Mass
993.634 Da
Monoisotopic Mass
993.634 Da
Topological Polar Surface Area
358.000 Ų
Heavy Atom Count
70
Formal Charge
0
Complexity
1830.000
Isotope Atom Count
0
Defined Atom Stereocenter Count
10
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
The total count of all stereochemical bonds
0
Covalently-Bonded Unit Count
1
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Application Protocols
No item-specific, vendor-validated application protocols are provided in the Product Data for ABT-510.
General example workflows (literature/general, to be adapted and validated by the user):
Endothelial tube formation assay:
Prepare peptide stock (e.g., in DMSO or water) and dilute into assay medium to a test range (e.g., 0.1–20 µM). Keep vehicle constant across conditions.
Seed endothelial cells on Matrigel or collagen; treat immediately with peptide.
Image network formation after 4–8 h and quantify branch points vs. controls.
Receptor binding (plate-based):
Coat plates with recombinant receptor or capture antibody. Block with BSA.
Incubate with a fixed concentration of labeled peptide or peptide followed by detection reagent; perform competition with unlabeled peptide to assess specificity.
Read fluorescence/chemiluminescence and fit binding/competition curves.
Signaling readouts:
Treat cells with peptide for defined times (e.g., 5–60 min) and analyze phospho-proteins by Western blot or ELISA.
These are general templates; optimize concentration, timing, and buffer conditions for your specific model. Always include negative controls (vehicle, scrambled peptide) and positive controls where appropriate.
Biological Roles
Scope: Biological context is provided as literature/general background to guide experimental design. No medical or clinical claims are made.
Literature/general background:
Thrombospondin-1 (TSP-1) is an endogenous matricellular protein that modulates cell adhesion, migration, and angiogenic signaling. Short synthetic peptides have been designed to mimic specific TSP-1 epitopes.
ABT-510 is widely referenced as a TSP-1–mimetic research peptide that engages angiogenesis-related pathways, including interactions reported with CD36 and downstream signaling cascades that influence endothelial behavior in vitro (literature).
Experimental systems: Studies commonly assess effects on endothelial cell tube formation, chemotaxis, adhesion, and apoptosis markers in culture, and examine signaling outputs such as MAPK/AKT pathway modulation and nitric oxide signaling (literature).
Mechanistic probes: The peptide is used alongside pathway inhibitors or receptor knockdown/knockout to dissect the dependence of phenotypic outcomes on specific receptors/co-receptors (e.g., CD36, integrins) (literature).
Biophysical aspects: Reported sequences incorporate modifications that enhance proteolytic stability and receptor affinity, enabling use in serum-containing media for limited durations (literature). Users should confirm stability and adsorption behavior under their own assay conditions.
Item-specific biological role statements are not provided in the Product Data; consult the CoA/Spec Sheet for any validated targets or assay results associated with this lot.
Buffer Applications
ABT-510 is a bioactive research peptide and is not itself a buffering agent. Therefore, classical buffer system design (e.g., phosphate, HEPES, Tris pH ranges) is not directly applicable.
Practical notes for use of buffers with peptides (literature/general):
Choose biologically compatible buffers (e.g., PBS, HEPES, or serum-free assay buffers) that maintain peptide stability and solubility. Avoid high concentrations of divalent cations or surfactants unless required by the assay, as these can influence peptide aggregation or receptor interactions.
Adjust pH to optimize solubility and receptor engagement; mildly acidic conditions can help dissolve basic peptides, while mildly basic buffers assist acidic sequences.
Include a low level of carrier protein (e.g., 0.01–0.1% BSA) when working at very low concentrations to minimize adsorption to plastics and glass.
Filter-sterilize final working solutions through 0.22 µm membranes if sterility is required for cell-based assays.
Item-specific buffer recommendations are not provided in the Product Data; refer to CoA/Spec Sheet or internal method development.
Green Alternatives
Relevance: ABT-510 is a bioactive research peptide rather than a bulk solvent or commodity reagent; “green alternatives” primarily concern solvent choices and handling during dissolution and assays.
Greener practice options (literature/general):
Prefer water or buffered saline as primary media whenever solubility permits; minimize DMSO/DMF content in final assay solutions.
Use small-volume, high-concentration DMSO stocks (e.g., 10–50 mM) to reduce organic solvent usage, then dilute into aqueous buffer.
Select low-toxicity, readily recyclable lab consumables when feasible and consolidate peptide manipulations to reduce plastic waste.
Employ microscale assay formats (96–384 well) to decrease total chemical use without sacrificing statistical power.
Trade-offs (general):
Aqueous-only dissolution may be limited by hydrophobic residues leading to aggregation; a modest DMSO co-solvent fraction can be required to maintain monomeric peptide.
Lower organic content is environmentally favorable but may reduce solubility or alter adsorption to plastics; mitigate by adding inert carriers (e.g., 0.01–0.1% BSA) and pre-wetting pipette tips.
Comparison snapshot (general):
Water/buffer: Greenest, biocompatible; limited solubility for hydrophobic sequences.
DMSO: Excellent solvency; environmental/health considerations require minimization and proper waste handling.
DMF: Useful for initial dissolution but less desirable in biological contexts; avoid in final assay media.
Pharmaceutical Uses
No pharmaceutical or clinical usage claims are made for this product. It is provided strictly for research use only, as indicated in the Product Data.
Context (literature/general, non-clinical):
In the scientific literature, TSP-1–mimetic peptides such as ABT-510 have been used as research tools to study pathways relevant to angiogenesis and extracellular matrix signaling. Any discussion in the literature of clinical contexts is outside the scope of this catalog entry and must not be inferred as guidance for use.
This product is not described as a pharmacopeial-grade material, excipient, or GMP-manufactured substance in the Product Data.
Item-specific information:
Regulatory/compendial status: Not specified for this item; refer to CoA/Spec Sheet.
Recommended applications in drug product manufacturing or formulation: Not applicable; research use only.
Physical Properties
Item-specific properties (from Product Data):
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
Not specified for this item; refer to CoA/Spec Sheet for any of the following if applicable: melting point, solubility limits, water content, residual solvents, optical rotation, counterion identity, peptide purity method, or peptide content by AAA.
Literature/general properties for guidance (not specifications for this item):
Form: Synthetic peptides are typically supplied as lyophilized solids or films to enhance stability.
Solubility (general): Many research peptides dissolve in water, dilute aqueous buffers, or polar aprotic solvents (e.g., DMSO). If basic residues dominate, dissolution in acidic aqueous media (e.g., 0.1% TFA or acetic acid) may help. For hydrophobic sequences, co-solvents (DMSO, DMF) are often used, followed by dilution with buffer (literature).
pH behavior: Ionizable side chains confer pH-dependent solubility; adjusting pH away from the isoelectric point typically improves solubility (literature).
Stability: Lyophilized peptides are generally stable at ambient temperature when dry and protected from moisture and light; solutions are less stable and usually stored refrigerated or frozen (literature).
Quality and Grades
Item-specific grade/purity: Not specified for this item; refer to CoA/Spec Sheet for peptide purity (e.g., HPLC area %), identity confirmation (MS), counterion content, residual solvents, and water content.
General notes for peptide quality (literature/general):
Peptide purity reporting: Research peptides are commonly qualified by analytical RP-HPLC (area %) and ESI/ MALDI mass spectrometry. Some suppliers also provide amino acid analysis (AAA) for peptide content and Karl Fischer for moisture.
Counterions and salts: Trifluoroacetate (TFA) is common from RP-HPLC; alternative counterions (acetate, chloride) may be specified on the CoA. Counterion choice can impact bioassay performance and cell compatibility.
Modifications: End-group modifications (e.g., N-acetylation, C-amidation) and noncanonical residues (N-methylation, D-residues) can influence solubility, proteolytic stability, and receptor affinity. Verify exact sequence and modifications on the CoA before use.
Batch-to-batch consistency: Peptides can show minor lot variations in salt form and residual moisture; equilibrate to room temperature before opening, and record lot-specific assay performance.
Chromatography grade vs. bioactive grade: When chromatography-specific grades are claimed (e.g., HPLC grade solvents), low UV background is prioritized. For bioactive peptides, high chemical and sequence fidelity, low deletion sequences, and correct mass are critical.
Reaction and Applications
Item-specific applications: Not specified for this item; refer to CoA/Spec Sheet and catalog page.
Research applications (literature/general) relevant to ABT-510 as a TSP-1–mimetic peptide:
Cell signaling studies: Probe thrombospondin-1–associated pathways, especially interactions that modulate angiogenesis-related signaling (e.g., CD36 pathway) in endothelial and stromal cells.
Receptor/ligand binding assays: Evaluate peptide–receptor engagement using ELISA-type binding formats, SPR, BLI, or fluorescence polarization.
Functional cell assays: Assess effects on endothelial cell migration, tube formation, or adhesion under defined serum conditions; design concentration–response curves and compare to scrambled peptide controls.
Biochemical pathway interrogation: Combine with inhibitors or siRNA/CRISPR perturbations to map pathway dependencies; monitor downstream phosphorylation or transcriptional readouts by Western blot, qPCR, or reporter assays.
In vitro stability profiling: Examine proteolytic stability in serum or cell media; use LC–MS to follow intact peptide over time.
Practical tips (general):
Prepare fresh working solutions; include carrier proteins (e.g., 0.1% BSA) to reduce adsorption losses for low-nanomolar dosing.
Validate activity with known positive controls and sequence-verified lots. Employ appropriate vehicle controls (e.g., DMSO ≤ assay limit).
Reaction Conditions
Traditional organic reaction conditions are not applicable, as ABT-510 is a bioactive peptide used in biochemical and cell-based experiments rather than a transformation reagent.
General experimental conditions for biological assays (literature/general):
Stock solutions: Prepare concentrated stocks in water, buffer, or DMSO depending on solubility. Common DMSO stocks are 10–50 mM; aqueous stocks vary by sequence. Filter-sterilize if required.
Working concentrations: Cell-based assays frequently explore 0.1–50 µM ranges, depending on assay sensitivity and receptor expression; fine-tune by titration and include appropriate vehicle controls (literature guidance, not item-specific).
Temperature and timing: Incubate at 37°C for live-cell assays or at room temperature/4°C for binding assays as dictated by the protocol; exposure times vary from minutes (acute signaling) to 24–72 h (phenotypic outcomes).
Assay media: Use serum-free or defined media during treatment windows to reduce peptide sequestration by serum proteins; alternatively, confirm activity in serum-containing media if relevant to the model.
Stability precautions: Avoid repeated freeze–thaw of stock solutions; aliquot upon initial reconstitution. Protect from light if fluorescently labeled derivatives are used.
Item-specific, validated conditions are not provided in the Product Data and should be established empirically.
Safety and Handling
Item-specific hazard data (from Product Data):
GHS Classification: Not specified for this item; refer to SDS.
Signal Word: Not specified for this item; refer to SDS.
H-Statements: Not specified for this item; refer to SDS.
Pictograms: Not specified for this item; refer to SDS.
General laboratory safety guidance (literature/general):
Handle peptides as research chemicals in a chemical fume hood or well-ventilated area. Avoid inhalation of powders/aerosols and contact with skin/eyes.
Personal protective equipment (PPE): Lab coat, safety glasses or goggles, and appropriate chemical-resistant gloves. For weighing fine powders, consider a mask/respirator as per institutional policy.
Incompatibilities: Avoid strong oxidizers and strong acids/bases during storage; avoid moisture for lyophilized materials. Do not heat peptide solutions excessively or expose to repeated freeze–thaw cycles.
First aid overview: If inhaled, move to fresh air; if on skin, wash with soap and water; if in eyes, rinse cautiously with water for several minutes; if swallowed, rinse mouth and seek medical attention. Always follow institutional protocols.
Waste disposal: Dispose of solutions and contaminated materials in accordance with local regulations for organic chemical/biological research waste.
Authoritative source: Consult the product-specific SDS for definitive hazard classification, exposure controls, and spill/accident response.
Solvent Selection
Item-specific solvent recommendations: Not specified for this item; refer to CoA/Spec Sheet.
General guidance for peptide dissolution (literature/general):
Primary solvents: Water or aqueous buffers (pH 4–8) are preferred when the sequence is polar/charged. For hydrophobic sequences, start with a small volume of DMSO or DMF to create a concentrate, then dilute into buffer with vigorous mixing to avoid precipitation.
pH adjustment: Slightly acidic media (e.g., 0.1% acetic acid or 0.1% TFA) can protonate basic residues and enhance solubility; for acidic peptides, use basic buffers (e.g., 10–50 mM ammonium bicarbonate) to deprotonate acidic side chains.
Ionic strength: Low ionic strength buffers reduce salting-out; add salts only after complete dissolution.
Filtration: If sterile solutions are required, filter through 0.22 µm PVDF or PES after complete dissolution; avoid prolonged contact with strongly adsorptive membranes for hydrophobic peptides.
Compatibility notes: DMSO stocks are widely used for bioassays; keep final DMSO below cell-tolerated limits (commonly ≤0.1–0.5% v/v, assay-dependent). Avoid alcohols for highly hydrophobic peptides that tend to aggregate.
Comparison (general):
Water/buffer: biocompatible, ideal for immediate cell/biochem assays; limited for hydrophobic sequences.
DMSO: excellent solvating power; requires careful dilution to avoid precipitation and cytotoxicity.
DMF: strong solvent for initial dissolution; less common in biological assays, usually followed by buffer dilution.
Storage and Reconstitution
Item-specific storage from Product Data:
Storage Conditions: Room temperature.
Shipped In: Not specified for this item; refer to CoA/Spec Sheet.
General best practices for peptides (literature/general; do not supersede item-specific instructions):
Upon receipt, allow the container to equilibrate to room temperature before opening to prevent moisture condensation.
If supplied lyophilized: Store desiccated and protected from light. For long-term storage, many laboratories keep lyophilized peptides at 2–8°C or −20°C as a conservative practice. Follow any lot-specific guidance on the CoA.
Reconstitution: Choose solvent based on solubility (water/buffer for polar sequences; DMSO or DMF as co-solvents for hydrophobic sequences). Start with a small volume and gentle vortex/sonication. Adjust pH if needed.
Working solutions: After reconstitution, aliquot into single-use portions to avoid repeated freeze–thaw. Store aqueous aliquots at 2–8°C for short term (hours to days) and at −20°C or below for longer term; avoid multiple cycles. DMSO stocks are typically stable at −20°C for weeks to months when protected from moisture.
Label aliquots with concentration, solvent, and date. Inspect for precipitation or discoloration before use. Discard if contamination or degradation is suspected.
For definitive stability and storage limits, consult the product’s CoA/Spec Sheet and SDS.
Structure and Identity
Brief description: ABT-510 is a research peptide classified as a thrombospondin-1 (TSP-1)–mimetic small molecule/peptide tool compound used to study angiogenesis-related signaling in cells and biochemical systems (literature/general).
Item-specific (from Product Data):
SKU: A1039058
Product Name: Abt-510
CAS: 251579-55-2
PubChem CID: 6918562
Storage Conditions: Room temperature
Research Use Note: For research use only
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
InChIKey: Not specified for this item; refer to CoA/Spec Sheet. (Product Data string provided is not a standard InChIKey)
Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
Literature/general structural information (not item-specific specs):
Chemical class: Synthetic peptide (TSP-1 type I repeat–derived mimetic) (literature).
Structural features: Linear, N- to C-terminal peptide with engineered residues to enhance stability and receptor engagement; often N-acetylated and C-terminally amidated in reported studies (literature).
2D structural description: A linear polyamide chain bearing side-chain functionalities (aliphatic, aromatic, ionizable) arranged to mimic the CD36-binding epitope of thrombospondin-1 (literature).
Synthetic Utility
ABT-510 is a finished research peptide rather than a general-purpose synthetic building block; it is not typically used as a reagent or intermediate in organic synthesis.
Literature/general context for chemists:
Structural features: Linear peptide with engineered sequence motifs reflecting thrombospondin-1 epitopes; amide bonds and side chains provide multiple hydrogen-bonding and ionic interaction sites for receptor engagement.
Method of preparation (typical, literature): Solid-phase peptide synthesis (SPPS) using Fmoc/tBu strategy on an amide-generating resin to furnish C-terminal amide; optional N-terminal acetylation and side-chain deprotection/coupling steps for noncanonical residues. Final purification is usually by RP-HPLC with identity confirmed by MS.
Derivatization opportunities: Biotinylation, fluorescent labeling (FITC, TAMRA), or PEGylation can be introduced at the N-terminus or on engineered lysine residues for assay development, pull-downs, or imaging (literature). Such derivatives are distinct products and not implied for this item unless specified on its CoA.
Immobilization: Incorporation of a terminal handle (e.g., primary amine) allows coupling to NHS-activated surfaces for SPR/BLI or plate-based assays; care must be taken to preserve the bioactive epitope (literature).
Item-specific synthetic details or sequence are not provided in the Product Data; consult CoA/Spec Sheet if needed.
Target Specificity
Item-specific target details (antigen, receptor, species reactivity, clone/isotype) are not provided in the Product Data for ABT-510. No tested targets are claimed.
Literature/general context:
TSP-1–mimetic peptides like ABT-510 are commonly used to interrogate pathways associated with thrombospondin-1 interactions, including putative engagement with CD36 and related signaling partners in endothelial systems. The exact target profile and binding constants can vary by sequence variant, assay format, and experimental conditions and should be determined empirically for each lot and application.
Users should consult the CoA/Spec Sheet for any lot-specific binding data, if available, or perform in-house validation (e.g., SPR/BLI, ELISA, cell-based competition assays) to define specificity in their system of interest.
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