Acidic Ethanol Differentiation Solution (0.5%) - BioReagent,Biological Stain,for microscopy,0.5%

Cat. No.: A1507832

QUALITÉ & PURETÉ BioReagent, Biological Stain, for Microscopy, 0.5%

Synonyms

Acidic Ethanol Differentiation Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Vue d’ensemble

  Differentiation refers to the process of removing excess stain that has bound to tissues using specific solutions after tissue staining; the solution employed for this purpose is called a differentiation solution. In HE staining (Hematoxylin-Eosin staining) and other staining procedures, 1% hydrochloric acid-ethanol is commonly used as a differentiation solution. This is because the acid can disrupt the quinone structure of hematoxylin, causing the separation of tissues and pigments (thereby resulting in decolorization). Only after this step is eosin staining carried out can a clear distinction between the staining of cell nuclei and cytoplasm be guaranteed.

  The Acidic Ethanol Differentiation Solution (0.5%) is mainly composed of dilute acid, ethanol, etc. It is frequently used in HE staining and Masson trichrome staining, and is more suitable for tissues that require mild differentiation power. It is an extremely important auxiliary reagent. This reagent is for scientific research use only and is not applicable for clinical diagnosis or other purposes.

Operating Procedures (for reference only)

1. Operate in accordance with the specific requirements of the experiment.

2. Generally, the differentiation process takes 2-5 seconds, and should be immediately terminated by rinsing with water or using a bluing solution.

Precautions

1. Store in a sealed container. Once opened, use it as soon as possible, as its active ingredients are volatile.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

3. After opening the reagent, use it promptly to avoid affecting the results of subsequent experiments.

Spécifications

Synonymes

Acidic Ethanol Differentiation Solution

Spécifications et pureté

BioReagent,Biological Stain,for microscopy,0.5%

Stabilité et stockage

Store at room temperature long term (24 months). Store in the dark.

Conditions de stockage de stockage

Protected from light,Room temperature

Expédié en

Normal

Grade

Biological Stain, BioReagent, pour la microscopie

Product Line

Produits biologiques

Propriétés chimiques et physiques

• Sensibilité: Light-sensitive

Foire aux questions

What does Biological Stain mean?

Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.