Alkyne tyramide - 10mM in DMSO , CAS No.1694495-59-4

CAS: 1694495-59-4 Cat. No.: A655029 Formule: C₁₃H₁₅NO₂ Poids moléculaire: 217.26 PubChem CID: 108021005

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne tyramide substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne tyramide also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins.

In Vitro

Yeast cells expressing Su9-APEX2 are incubated with each probe at 2.5 mM final concentration for half an hour, and the labeling reaction is initiated by the addition of 1 mM H2O2. Alkyne tyramide is the most reactive APEX2 substrate for labeling the yeast proteome. ?\nAPEX2-mediated Alkyne tyramide labeling in the yeast mitochondria is more effective at probe concentrations above 1 mM. ?\nAlkyne tyramide enables proteomic profiling in the mitochondrial matrix of intact yeast cells with exceptionally high specificity (94%), and offers higher coverage than the traditional APEX2 substrate biotin-phenol (BP). MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Specifications

Spécifications et pureté

10mM in DMSO

Mécanismes biochimiques et physiologiques

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne tyramide substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne tyramide also faci

Conditions de stockage de stockage

Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Noms et identifiants

• Isomères SMILES: C#CCCC(=O)NCCC1=CC=C(C=C1)O
• PubChem CID: 108021005
• Poids moléculaire: 217.26