Anticorps DBF4 recombinant - Anticorps primaire, specific to DBF4, Rabbit IgG

Cat. No.: Ab099212

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

WB

Host species

Lapin

Reactive species

Humain

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

L'anticorps de lapin anti-humain DBF4, recombinant, peut être utilisé pour les tests de laboratoire, etc.

Specifications

Product Name

Anticorps DBF4 recombinant - Anticorps primaire, specific to DBF4, Rabbit IgG

Synonymes

Anticorps de l'activateur de la kinase en phase S | Anticorps ASK | Anticorps CHIF | Anticorps Chiffon homologue A | Anticorps DBF4 | Anticorps homologue DBF4 (S. cerevisiae) | Anticorps homologue DBF4 | Anticorps DBF4 type zinc finger containing protein

Spécifications et pureté

Recombinant, ExactAb™, validé, voir COA

Mécanismes biochimiques et physiologiques

Sous-unité régulatrice de CDC7 qui active son activité kinase, jouant ainsi un rôle central dans la réplication de l'ADN et la prolifération cellulaire. Nécessaire à la progression de la phase S. Le complexe CDC7-DBF4A phosphoryle sélectivement la sous-un

Espèce hôte

Lapin

Spécificité

DBF4

Immunogène

A synthetic peptide derived from human DBF4 (AA 2-55)

Contrôle positif

WB: Jurkat lysates

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Stockage et expédition

Forme

Liquide

Conditions de stockage de stockage

Conserver à -20°C, éviter les congélations et décongélations répétées

Expédié en

Glacière + blocs de glace

Stabilité et stockage

Stocker à 4°C à court terme (1-2 semaines). Stocker à -20°C à long terme (24 mois). Dès réception, il est recommandé d'aliquoter. Éviter les cycles de congélation/décongélation.

Images

Western Blot: Recombinant DBF4 Antibody (Ab099212)

Recombinant DBF4 Antibody (Ab099212) - Western Blot
All lanes: Recombinant DBF4 Antibody (Ab099212) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 77 kDa
Observed band size: 73, 75 kDa
The DBF4 protein contains a DNA-binding motif, which affects its migration in Western blot experiments. Its expression pattern aligns with expectations.
Exposure time: 50.0 s

Application

• Application: Dilution info
• WB:

  1/1000-1/2000 ;

Cibles associées (humaines)

DBF4 Tbio Protein DBF4 homolog A (0 Activities)

Discover Target: DBF4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

DBF4 Tbio Activator of S phase kinase DBF4 (1 Activities)

Discover Target: DBF4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant DBF4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.