GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.See COA
Recombinant, ExactAb™, KD Validation, Validated, See COA ExactAb™,KD Validation,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human MBD1 Antibody, Recombinant, could be used for WB and so on.
Specifications
Product Name
Recombinant MBD1 Antibody - Primary antibody, specific to MBD1, Rabbit IgG
Recombinant, ExactAb™, KD Validation, Validated, See COA
Biochemical and Physiological Mechanisms
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts
Host species
Rabbit
Specificity
MBD1
Immunogen
A synthetic peptide derived from human MBD1 (AA 550-605).
Positive Control
WB: HeLa, MCF7, Hep G2, HEK293 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, KD Validation, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MBD1 Antibody (Ab114408) - Western Blot All lanes: Recombinant MBD1 Antibody (Ab114408) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (AP132P) at 1/20000 dilution
Predicted band size: 67 kDa Observed band size: 74, 81 kDa. Recombinant MBD1 Antibody (Ab114408) recognizes two isoforms of MBD1. Exposure time: 36.1 s
Recombinant MBD1 Antibody (Ab114408) - Western Blot All lanes use Recombinant MBD1 Antibody (Ab114408) at 1/1000 dilution for 1 hour at room temperature.
Recombinant MBD1 Antibody (Ab114408) - Western Blot All lanes: Recombinant MBD1 Antibody (Ab114408) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (AP132P) at 1/20000 dilution
Predicted band size: 67 kDa Observed band size: 74, 81 kDa. Recombinant MBD1 Antibody (Ab114408) recognizes two isoforms of MBD1. Exposure time: 36.1 s
Application
Application
Dilution info
WB
1/1000-1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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