APN-CHO

Cat. No.: A487948

Synonyms

3-(4-formylphenyl)propiolonitrile, APN-aldehyde

Storage

Store at 2-8°C

Shipped In

Wet ice

Vue d’ensemble

Description

APN-Aldehyde is a bifunctional crosslinker for introduction of aldehyde into biomolecules. The coupling can be performed with high selectivity in biological medium using mild reaction conditions.

Preparation instructions

Standard protein labeling procedure (cysteine labeling).Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).Incubate at room temperature for 2 hours.If necessary, purify the protein-aldehyde conjugate using size exclusion chromatography or ultrafiltration.The conjugate can be readily coupled with hydrazides/hydrazones/hydroxylamines using standard protocols.

Specifications

Synonymes

3-(4-formylphenyl)propiolonitrile, APN-aldehyde

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.