B022 - Moligand™, ≥99% , CAS No.1202764-53-1

CAS: 1202764-53-1 Cat. No.: B649544 Formule: C₁₉H₁₆ClN₅OS Poids moléculaire: 397.88

GRADE & PURITY Moligand™, ≥99%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Vue d’ensemble

B022 is a potent and selective NF-κB-inducing kinase (NIK) inhibitor ( K i of 4.2 nM; IC 50 =15.1 nM). B022 protects liver from toxin-induced inflammation, oxidative stress, and injury

In Vitro

B022 (0-5 μM; 12 hours; Hepa1 cells) treatment suppresses NIK-induced p52 formation in a dose-dependent manner. B022 (0-5 μM; 12 hours; Hepa1 cells) treatment for 8 h completely blocks NIK-induced expression of TNF-a, IL-6, iNOS, CCL2, and CXCL5. B022 prevents NIK- or H2O2-induced β cell death and also ameliorates streptozotocin (STZ)-induced β cell death and hyperglycemia. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: Hepa1 cells Concentration: 0 μM, 0.5 μM, 5 μM Incubation Time: 12 hours Result: Suppressed NIK-induced p52 formation in a dose-dependent manner. RT-PCRCell Line: Hepa1 cells Concentration: 0 μM, 0.5 μM, 5 μM Incubation Time: 12 hours Result: Dose-dependently blocked NIK-induced expression of chemokines, cytokines, and iNOS in these cells. Completely blocked NIK-induced expression of TNF-a, IL-6, iNOS, CCL2, and CXCL5.

In Vivo

B022 (30 mg/kg; intravenous injection; twice a day; for 10 days; STOP-NIK male mice) treatment inhibits NIK-triggered liver inflammation and injury in STOP-NIK mice infected with cre adenoviruses . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: STOP-NIK male mice (8 weeks) infected with Ad-cre Dosage: 30 mg/kg Administration: Intravenous injection; twice a day; for 10 days Result: Completely prevents the lethal effect of abnormally high levels of hepatic NIK in mice. Inhibited the majority of the deteriorating effects of aberrant activation of hepatic NIK.

Form:Solid

IC50& Target:Ki: 4.2 nM (NF-κB-inducing kinase (NIK))

Specifications

Spécifications et pureté

Moligand™, ≥99%

Conditions de stockage de stockage

Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

Moligand™

Type d'action

INHIBITOR

Pureté

≥99%

Noms et identifiants

Poids moléculaire

397.88

Propriétés chimiques et physiques

• Solubilité: DMSO : 250 mg/mL (628.33 mM; Need ultrasonic)