BI8622 - 10mM in DMSO , CAS No.1875036-74-0

CAS: 1875036-74-0 Cat. No.: B655041 Formule: C₂₅H₂₆N₆O Poids moléculaire: 426.51 PubChem CID: 138377590

QUALITÉ & PURETÉ 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC 50 of 3.1 μM

In Vitro

BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC 50 value of 6.8 μM in HeLa cells. BI8622 suppresses colony formation of Ls174T cells with estimated IC 50 value of 8.4 μM. BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells. BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cycle AnalysisCell Line: Ls174T cells Concentration: 0 μM, 5 μM,10 μM, 15 μM, 20 μM Incubation Time: 0-4 days Result: Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. Western Blot AnalysisCell Line: HeLa cells Concentration: 0 μM, 10 μM, 20 μM Incubation Time: 16 hours Result: Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.

IC50& Target:IC50: 3.1 μM (HUWE1)

Spécifications

Spécifications et pureté

10mM in DMSO

Mécanismes biochimiques et physiologiques

BI8622 is a specific inhibitor of the ubiquitin ligase HUWE1 with an IC 50 of 3.1 μM.

Conditions de stockage de stockage

Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Type d'action

INHIBITOR

Noms et identifiants

PubChem CID

138377590

Poids moléculaire

426.51