BT57 Reagent - BioReagent,suitable for mass spectrometry (MS),≥99%(HPLC)

Cat. No.: B1523261

GRADE & PURITY BioReagent, Suitable for mass spectrometry (MS), ≥99%(HPLC)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Protein/Antibody Characterization

Vue d’ensemble

BT57 is an all-in-one reagent for rapid denaturation, reduction and alkylation. In biopharmaceutical and proteomics research, native proteins with three-dimensional structures undergo denaturation, reduction, alkylation, desalting, and subsequent enzymatic digestion. Traditional denaturants such as urea and guanidine hydrochloride can cause chemical modifications to proteins, such as carbamylation. In addition, conventional alkylating reagents require protection from light. BT57 all-in-one reagent enables one-step addition and rapidly completes protein denaturation, reduction and alkylation. It is suitable for biopharmaceutical and simple protein sample research. Physical Form: Lyophilized powder containing 0.8mg BT Surfactant, TCEP, ABC and CAA. Reconstitution: Reconstitute with 205 μL of ultrapure water. pH Range: pH 7–9 for protein denaturation. The reagent degrades and precipitates in solution at pH 2–4. Usage: Add 10 μL of BT57 reagent to 10 μL (50–100 μg) protein sample, mix well. Incubate in a dry bath at 60 °C for 20 min, or at 95 °C for 10 min.

Specifications

Spécifications et pureté

BioReagent,suitable for mass spectrometry (MS),≥99%(HPLC)

Conditions de stockage de stockage

Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

Adapté à la spectrométrie de masse (MS), BioReagent

Pureté

≥99%(HPLC)