CD25 Rat mAb (AF405) - Anticorps primaire, specific to CD25

Cat. No.: Ab211655
DISPONIBLE À COMMANDE
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:401nm ? Fluorescence excitation maximum at 401 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:422nm ? Fluorescence emission maximum at 422 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
conseil
Reactive species
Souris
Isotype
Rat IgG1
Clone Number
PC-61.5.3
Conjugation
AF405
Size
Allemagne (EU)
USA*
Price
Qty
10μg
Ab211655-10μg
3 En stock
62,39€
25μg
Ab211655-25μg
2 En stock
123,13€
100μg
Ab211655-100μg
1 En stock
303,62€
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, Ex:401nm, Em:422nm, 0.5 mg/mL Em:422nm,Ex:401nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Vue d’ensemble

Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry

Specifications

Product Name
CD25 Rat mAb (AF405) - Anticorps primaire, specific to CD25
Synonymes
Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor sub
Spécifications et pureté
ExactAb™, Validated, Ex:401nm, Em:422nm, 0.5 mg/mL
Espèce hôte
conseil
Spécificité
CD25
Immunogène
IL-2-dependent cytolytic mouse T cell clone B6.1
Contrôle positif
Flow: C57BL/6 mouse splenocytes
Conjugation
AF405
Excitation(nm)
Violet Laser(405nm)
Émission (nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grade
Em:422nm, Ex:401nm, ExactAb™, Validated
Type de chaîne lumineuse
lambda
Méthode de purification
Protein A purified
Concentration
0.5 mg/mL
Stockage et expédition
Conditions de stockage de stockage
Store at 2-8°C,Protected from light,Do not freeze
Expédié en
Wet ice,Do not freeze
Stabilité et stockage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

CD25 Rat mAb (AF405) (Ab211655) - Flow Cytometry
Con A-stimulated (2 days) C57BL/6 mouse splenocytes (right panel) stained with CD25 Rat mAb (AF405) (Ab211655) (red) compared to an isotype control (blue). Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Application
ApplicationDilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Cibles associées (humaines)
IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateArticle
ZJ24F1216054Certificate of AnalysisJul 09, 2026 Ab211655
ZJ24F1216055Certificate of AnalysisJul 09, 2026 Ab211655
ZJ24F1216056Certificate of AnalysisJul 09, 2026 Ab211655
FAQ et articles
Calculateurs de solution
Avis

Avis des clients

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:401nm grade guide → View Em:422nm grade guide →

Produits apparentés

CD25 Rat mAb - Anticorps primaire, for In vivo regulatory T cell depletion,Flow cytometry various applications, from Mouse, generated in Rat, specific to CD25, IgG1
Rat IgG - Anticorps de contrôle isotype, IgG
Goat Anti-Rat IgG H&L Antibody - Anticorps secondaire, Goat IgG
Goat Anti-Rat IgG H&L (Biotin) - Anticorps secondaire, Goat IgG
Rat IgG (Biotin) - Anticorps de contrôle isotype, IgG
Rat IgG (PE) - Anticorps de contrôle isotype, IgG
Rat IgG (FITC) - Anticorps de contrôle isotype, IgG
Rat IgG (APC) - Anticorps de contrôle isotype, IgG
CD25 Rat mAb (APC) - Anticorps primaire, specific to CD25, IgG1
CD25 Rat mAb (FITC) - Anticorps primaire, specific to CD25, IgG1
CD25 Rat mAb (Biotin) - Anticorps primaire, specific to CD25, IgG1
CD25 Rat mAb (PE) - Anticorps primaire, specific to CD25, IgG1
Rat IgG2a-kappa, anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD, Rat IgG2a
Rat IgG2b-kappa, anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD, Rat IgG2b
Rat IgG1-kappa - Anticorps de contrôle isotype, Rat IgG1
Rat IgG (AF405) - Anticorps de contrôle isotype, IgG
Rat IgG (AF647) - Anticorps de contrôle isotype, IgG
Rat IgG (AF700) - Anticorps de contrôle isotype, IgG
CD25 Rat mAb (PE-Cy5) - Anticorps primaire, specific to CD25
CD25 Rat mAb (AF647) - Anticorps primaire, specific to CD25
CD25 Rat mAb (AF555) - Anticorps primaire, specific to CD25
Rat IgG (AF555) - Anticorps de contrôle isotype
Rat IgG2b-kappa (Biotin), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2b-kappa (APC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2b-kappa (FITC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2b-kappa (AF555), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2b-kappa (AF647), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2a-kappa (AF405), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2a-kappa (AF647), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2a-kappa (FITC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2a-kappa (APC), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2b-kappa (AF700), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2b-kappa (AF405), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG2a-kappa (AF700), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG (PE-Cy5) - Anticorps de contrôle isotype
Rat IgG (APC-Cy7) - Anticorps de contrôle isotype
Rat IgG2a-kappa (AF555), anti-Spike RBD - Anticorps de contrôle isotype, specific to Spike RBD
Rat IgG (AF488) - Anticorps de contrôle isotype
CD25 Rat mAb (AF488) - Anticorps primaire, specific to CD25
Rat IgG (PE-Cy7) - Anticorps de contrôle isotype

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