CD83 Rat mAb - Anticorps primaire, specific to CD83, Rat IgG1

Cat. No.: Ab095443

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, ELISA, Flow Cytometry, Functional Assay

Host species

conseil

Reactive species

Souris

Isotype

Rat IgG1

Clone Number

Michel-17

Conjugation

Unconjugated

Vue d’ensemble

Rat anti Mouse CD83 Antibody, Monoclonal (Michel-17), could be used for in vivo CD83 engagement, in vitro CD83 blockade, Flow, ELISA and so on.
Application:
in vivo CD83 engagement: Use at an assay dependent concentration.
in vitro CD83 blockade: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Protein Function
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Specifications

Product Name

CD83 Rat mAb - Anticorps primaire, specific to CD83, Rat IgG1

Synonymes

BL11 | BL11CD83 antigen | CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) | CD83 molecule | CD83 | Cell surface protein HB15 | cell-surface glycoprotein | HB15 | HB15hCD83 | B cell activation 45kDa cell surface glycoprotein Ig superfami

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Espèce hôte

conseil

Spécificité

CD83

Immunogène

CD83-Ig fusion proteins

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Rat IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD83 Rat mAb (Ab095443)

CD83 Rat mAb (Ab095443) - Flow Cytometry
Flow Cytometry analysis of 1 ng/mL LPS stimulated mouse splenocytes cells (72h) labelling CD83 with CD83 Rat mAb (Ab095443) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD83 Rat mAb (Ab095443)

CD83 Rat mAb (Ab095443) - Flow Cytometry
Flow Cytometry analysis of 1 ng/mL LPS stimulated mouse splenocytes cells (72h) labelling CD83 (red) with CD83 Rat mAb (Ab095443) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

• in vivo CD83 engagement:

  Use at an assay dependent concentration.;

• in vitro CD83 blockade:

  Use at an assay dependent concentration.;

Foire aux questions

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD83 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.