CMLD-2 - 10mM in DMSO , CAS No.958843-91-9

CAS: 958843-91-9 Cat. No.: C654542 Formule: C₃₁H₃₁NO₆ Poids moléculaire: 513.58

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

CMLD-2, an inhibitor of HuR-ARE interaction , competitively binds HuR protein disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs ( K i =350 nM). CMLD-2 induces apoptosis exhibits antitumor activity in different cancer cells as colon, pancreatic, thyroid and lung cancer cell lines. Hu antigen R (HuR) is an RNA binding protein, can regulate target mRNAs stability and translation

In Vitro

CMLD-2 (1-75 μM; 24-72 h) inhibits thyroid cancer cell viability. CMLD-2 (20-30 μM; 24-48 h) activates caspases and induces apoptotic cell death in H1299 and A549 cells. CMLD-2 (30 μM; 24-48 h) induces G1 cell cycle arrest and mitochondrial perturbation in H1299 and A549 cell. CMLD-2 (30 μM; 24-48 h) reduces expression of HuR and HuR-regulated mRNAs and proteins in H1299 cells. CMLD-2 (35 μM; 72 h) decreases directional migration capability in SW1736, 8505C, BCPAP and K1 cells. CMLD-2 induces a strong decrease of MAD2 mRNA levels in SW1736, 8505C, BCPAP and K1 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: SW1736, 8505C, BCPAP and K1 cells Concentration: 1, 5, 10, 25, 35, 50, 75 μM Incubation Time: 24, 48, 72 hours Result: Reduced the viability of all the four cell lines when used at 35, 50 and 75 μM concentration and at different time points. Apoptosis AnalysisCell Line: H1299, A549, H1975, HCC827, MRC-9 and CCD16 cells Concentration: 20, 30 μM Incubation Time: 24, 48 hours Result: Marked activated the caspase-9 and -3 in lung tumor cells. Induce the cleavage of PARP in lung tumor cells. Significantly increased the annexin-V-positive staining in lung tumor cells. Cell Cycle AnalysisCell Line: H1299, A549, MRC-9 and CCD16 cells Concentration: 30 μM Incubation Time: 24, 48 hours Result: Induced greater G1 phase cell cycle arrest in H1299 and A549 cells than in MRC-9 and CCD16 cells. Western Blot AnalysisCell Line: H1299, A549, H1975, HCC827, CCD16 and MRC-9 cells Concentration: 20, 30 μM Incubation Time: 24, 48 hours Result: Diminished protein expression of HuR, Bcl-2, Cyclin E and Bcl-XL and increased expression of p27 and BAX in lung tumor cells.

IC50& Target:HuR

Specifications

Spécifications et pureté

10mM in DMSO

Mécanismes biochimiques et physiologiques

CMLD-2, an inhibitor of HuR-ARE interaction , competitively binds HuR protein disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs ( K i =350 nM). CMLD-2 induces apoptosis exhibits antitumor activity in different cancer cel

Conditions de stockage de stockage

Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Type d'action

INHIBITOR

Noms et identifiants

• Poids moléculaire: 513.58

Foire aux questions

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 958843-91-9, the molecular formula is C31H31NO6, and the molecular weight is 513.58 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.