DAPI staining solution (ready to use) , CAS No.28718-90-3

CAS: 28718-90-3 Cat. No.: D598342 Formule: C₁₆H₁₅N₅·2HCl Poids moléculaire: 350.25 Beilstein Registry Number: 4894417 Numéro CE: 249-186-7 PubChem CID: 160166

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Vue d’ensemble

Product introduction：

DAPI is a nuclear staining reagent that can stain DNA. It releases blfluorescence after embedding double stranded DNA, and the brightness is enhanced about 20 times. DAPI is often used for apoptosis detection, and it is observed by fluorescence microscope or detected by flow cytometry after staining. DAPI has a high photobleaching tolerance and can be used to detect yeast mitochondrial DNA, chloroplast DNA, viral DNA and stained DNA. Take adherent cells (96 well plate) as an example, 100 per well μ L staining working solution, 10 ml can be used for the staining of 100 wells.

Product parameters：

Ex/em (bound DNA) =360/460 nmcas No.: 28718-90-3 molecular weight: 350.3 molecular formula: c16h17cl2n5

Matters needing attention：

1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. the concentration of this dyeing solution is optimized to meet the needs of various conventional dyeing. If you need to adjust the concentration, please select D489987 or D106471 ，and configure the appropriate working fluid concentration by yourself. 3. DAPI is harmful to human body. Please wear experimental clothes and disposable gloves for operation, and pay attention to proper protection. 4. fluorescent dyes have quenching problems. Please try to avoid light to slow down quenching.

Scope of application：

Nuclear staining

Instruction：

For cell or tissue samples, after fixation, appropriate washing to remove the fixative. If immunofluorescence staining is performed, DAPI staining is performed after staining. If no other staining is required, subsequent DAPI staining is performed directly. 1.For adherent cells ( remove the medium in the orifice plate ) or tissue sections, add a small amount of DAPI staining solution and cover the sample. For suspension cells, at least 3 times the volume of the sample to be stained was added and mixed. 2.Cells were cultured at room temperature for 10-20 min. 3.The cells were washed twice with PBS or appropriate buffer, 3-5 min each time, and 50 μL PBS was added after washing to prevent the cells from drying out. 4.Cells were observed under a fluorescence microscope with a filter with 360 nm excitation wavelength and 460 nm emission wavelength.

Specifications

Conditions de stockage de stockage

Protected from light,Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Noms et identifiants

Pubchem Sid

508817316

Sourires canoniques

C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl

IUPAC Name

2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide;dihydrochloride

InChIKey

FPNZBYLXNYPRLR-UHFFFAOYSA-N

INCHI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

Isomères SMILES

C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl

WGK Allemagne

3

PubChem CID

160166

Poids moléculaire

350.25

Beilstein

4894417

Reaxy-Rn

4894417

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Classe Indoles and derivatives
      • Subclass Indoles

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Classe: Indoles and derivatives
• Subclass: Indoles
• Intermediate Tree Nodes: Not available
• Direct Parent: 2-phenylindoles
• Alternative Parents: Phenylpyrroles Benzene and substituted derivatives Heteroaromatic compounds Carboximidamides Carboxamidines Azacyclic compounds Organopnictogen compounds Hydrochlorides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: 2-phenylindole - 2-phenylpyrrole - Monocyclic benzene moiety - Substituted pyrrole - Benzenoid - Pyrrole - Heteroaromatic compound - Amidine - Carboxylic acid amidine - Azacycle - Carboximidamide - Organopnictogen compound - Organonitrogen compound - Hydrocarbon derivative - Organic nitrogen compound - Hydrochloride - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as 2-phenylindoles. These are indoles substituted at the 2-position with a phenyl group.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Mécanismes d'action

Propriétés chimiques et physiques

• Poids moléculaire: 350.200 g/mol
• Hydrogen Bond Donor Count: 7
• Hydrogen Bond Acceptor Count: 2
• Rotatable Bond Count: 3
• Exact Mass: 349.086 Da
• Monoisotopic Mass: 349.086 Da
• Topological Polar Surface Area: 116.000 Ų
• Heavy Atom Count: 23
• Formal Charge: 0
• Complexity: 413.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 3

XLogP3