5X for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product Introduction: GelRed is a novel nucleic acid stain. This unique oil-soluble macromolecule is non-volatile, non-inhalable, and cannot penetrate cell membranes to enter living cells. Additionally, it exhibits no mutagenicity at the gel-staining concentration. GelRed is characterized by its safety in use and high sensitivity in detection. It can be used as a staining agent for various nucleic acid electrophoresis applications and is suitable for staining nucleic acids of all fragment sizes. GelRed is an ideal alternative to ethidium bromide (EB). DNA Loading Buffer (5X, GelRed) needs to be mixed with the sample for staining. Composition: 10 mM Tris-HCl, 5 mM EDTA, pH 7.6, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, GelRed Nucleic Acid Stain. Usage Instructions: 1. Use in combination with Aladdin DNA Marker. 2. Mix 4 μl of sample (<500 ng) with 1 μl of DNA Loading Buffer (5X, GelRed). Allow the mixture to stand at room temperature for 2 minutes before loading. 3. It is recommended to use 1.0-2.0% agarose gel, with a voltage of 4-10 V/cm. Perform electrophoresis using 1x TAE (preferred) or 0.5x TBE buffer. Note: Replace the electrophoresis buffer promptly and use freshly prepared gels to achieve optimal results. 4. No additional stains are required in the agarose gel. After electrophoresis, the bands can be directly visualized under UV light. The stain is compatible with commonly used UV transilluminators.
Specifications
Synonyms
Red Fluorescent DNA Loading Buffer (5X) | 5xDNA Loading Buffer with GelRed
Specifications & Purity
5X
Stability And Storage
Store at 4 ℃ for 36 months. Store at -20 ℃ for 60 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage
Store at -20°C, Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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