APT (All Purpose Tween) Agar (Powder)

Directions per Liter:

Dissolve 58.43grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).

Component	g/liter
Yeast Extract .	7.5
Dextrose	10
Casein Digest Peptone.	10
Sodium Citrate	5
Potassium Phosphate	5
Sodium Chloride	5
Tween 80	0.2
Sodium Carbonate	1.25
Magnesium Sulfate	0.8
Magnesium Chloride	0.14
Ferrous Sulfate	0.04
Agar	13.5
	Total: 58.43g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.