Laboratory Protocols & SOPs Library

Coelenterazine Stock Solution for In Vivo Use

· Published 6 août 2025 · Updated 6 août 2025

Instructions

1. Add 5 mg coelenterazine (Aladdin Catalog C131248) to 1 ml of acidified methanol (add 50 µl of concentrated HCl to 10 ml methanol).

2. Aliquot and store at -80°C for up to 4 weeks.

3. For use, thaw tube and add 20 µl of coelenterazine stock solution to 130 µl DPBS and inject immediately.

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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Aladdin Scientific. "Coelenterazine Stock Solution for In Vivo Use" Aladdin Knowledge Base, updated 6 août 2025. https://www.aladdinsci.com/eu_fr/faqs/coelenterazine-stock-solution-for-in-vivo-use-en.html

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