To understand the difference between the action of non-depolarizing and depolarizing types of muscarinic drugs, and to learn the rabbit drooping head test method for muscarinic drugs.
Operation method
intramuscular injection
Principle
Skeletal muscle relaxants are divided into two categories: depolarizing muscarinic agents and non-depolarizing muscarinic agents. Depolarizing muscarinic agents are N2 receptor agonists, and non-depolarizing muscarinic agents are N2 receptor blocking agents, both of which can produce neuromuscular junction blocking effects, but the blocking modes are different, and the characteristics of the effects are not the same.
Materials and Instruments
Rabbits Move 1. 2 rabbits were taken, labeled, weighed, and observed for body position. The rabbits were weighed and observed for body position, respiration, neck and limb muscle tone. The rabbits were weighed and observed for body position, respiration, neck and limb muscle tone, etc. Then, atropine solution 0.5 mL was injected intramuscularly and waited for about 10 min. Caveat 1. When injecting muscarinic drugs, try to inject them slowly and at a constant speed of about 0.5 mL/min, so as to accurately measure the dose of the drooping head, and to facilitate the observation of the development of the action of muscarinic drugs. 2. Inject neostigmine quickly after the rabbits show paralysis, otherwise it is not easy to rescue. If the rescue effect is not good, the dosage of neostigmine can be increased. Common Problems How do pancuronium bromide and succinylcholine affect rabbits? Does neostigmine act differently on the two drugs? Why? For more product details, please visit Aladdin Scientific website.
Atropine sulfate solution; pancuronium bromide solution; succinylcholine chloride solution; neostigmine methyl sulfate solution
Syringe
2. Rabbit A: Draw 0.008% pancuronium bromide solution 0.5 mL/kg with a 2 mL syringe and inject it slowly intravenously at the rate of 0.5 mL/min. If the rabbit is found to have a tendency to droop its head, the injection should be suspended and the rabbit should be knocked lightly on the head. If the rabbit is still unable to raise its head at this time, record the amount of drug injected, which is the drooping dose of the rabbit. Then add 1 times the amount of pancuronium bromide, observe the changes of muscle tone and respiration of the rabbit's limbs, and immediately inject 0.05% neostigmine methyl sulfate solution 0.4 mL/kg intravenously, and observe the changes of the animal.
Rabbit B: As above, slowly inject 0.05% succinylcholine chloride solution 0.5 mL/kg intravenously and observe whether the rabbit has muscle tremor . Observe whether the rabbit has muscle tremor, head hanging or general paralysis. Immediately after the appearance of the phenomenon, inject 0.05% neostigmine methylsulfate solution 0.4 mL/kg intravenously, observe the changes of the animal and compare with rabbit A.
3. Results and analysis
Rabbit number Weight (kg) Drug Administration dose Post-drug performance armor plating Pancuronium bromide Pancuronium bromide Neostigmine ethyl succinylcholine Neostigmine