Construction of siRNA expression vector targeting EGFR gene
Protocols
Construction of siRNA expression vector targeting EGFR gene
·Reviewed·Published ·Updated ·8 min read
Summary
The constructed psj-EGFR vector was transfected into A549 cells using Lipofeetamine2000, and real-time quantitative fluorescence PCR, immunofluorescence and flow cytometry were applied to detect the expression of EGFR gene at the mRNA and protein levels, and tetramethyl azole blue colorimetric assay (MTT), and colony formation assay were used to observe the changes in cell growth.
For more product details, please visit Aladdin Scientificwebsite.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Cite this article
Aladdin Scientific. "Construction of siRNA expression vector targeting EGFR gene" Aladdin Knowledge Base, updated Dec 24, 2024. https://www.aladdinsci.com/us_en/faqs/construction-of-sirna-expression-vector-en.html
Was this article helpful?YesNo0 out found this helpful
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
