Dissolved amino acids: how to prepare a solution with 1 equivalent of NaOH?
Dissolved amino acids: how to prepare a solution with 1 equivalent of NaOH?
posted this at Jun 9, 2025 Updated at Jul 27, 2023
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Dissolved amino acids: how to prepare a solution with 1 equivalent of NaOH
Amino acids are often difficult to solubilize. A common technique is to use 1 molar equivalent (1eq) of sodium hydroxide (NaOH).
To dissolve compound in 1 equivalent of NaOH(aq.), the following formula can be used to calculate the volume of NaOH (aq) (VolNaOH) of concentration (ConcNaOH ) needed:
e.g for a product with a molecular weight of 197.13g, a mass of 5 mg, and using 100 mM NaOH:
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