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If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?

· Published 27 août 2024 · Updated 9 juin 2025

If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?

If the protein sample contains a relatively large number of dimers, the reducing agent DTT can be added to help open the dimers.

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Explore topics: Buffer protein Polymer heterobands Dimers

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Aladdin Scientific. "If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?" Aladdin Knowledge Base, updated 9 juin 2025. https://www.aladdinsci.com/eu_fr/faqs/i-need-to-add-doubling-buffer-to-open-the-dimers-en.html

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