LYAR Mouse mAb: Applications, Protocol & Usage Guide

LYAR Mouse mAb is a monoclonal antibody raised in mouse targeting the LYAR protein, supplied in PBS formulation and intended for use in research applications exploring LYAR's role in cell growth and nucleolar function.

Product Name

LYAR Mouse mAb

SKU

Ab350997

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) only

Supplier Reference

absea#KC-29864

Target Overview: What Is LYAR?

LYAR (Ly1 Antibody Reactive homolog) is a zinc finger nucleolar protein that plays important roles in cell growth regulation and ribosome biogenesis. It is primarily localized in the nucleolus and has been associated with the regulation of rDNA transcription, cell proliferation, and apoptosis. LYAR expression has been reported to be dysregulated in certain tumor contexts, making it a subject of interest in cancer biology and cell cycle research.

Research targeting LYAR with specific monoclonal antibodies enables precise detection and characterization of this protein in various experimental settings, supporting studies on nucleolar dynamics, cell growth signaling, and potential disease associations.

Antibody Specifications & Formulation

The LYAR Mouse mAb (Ab350997) is a monoclonal antibody produced in mouse. The product is supplied exclusively in PBS (phosphate-buffered saline) formulation without additional stabilizers or preservatives unless otherwise stated on the Certificate of Analysis. Key specifications include:

• Host Species: Mouse
• Antibody Format: Monoclonal IgG
• Formulation: PBS only
• Supplier Reference: absea#KC-29864
• SKU: Ab350997

Researchers should consult the product's Certificate of Analysis (CoA) for lot-specific details including concentration, purity, and validated applications, as these may vary between lots.

Recommended Protocol & Usage Guidelines

The following general protocol guidelines are provided for researchers using LYAR Mouse mAb. Always refer to the lot-specific datasheet for validated conditions and optimized dilutions.

Storage & Handling

• Store the antibody at –20 °C for long-term storage. Avoid repeated freeze-thaw cycles to preserve antibody integrity.
• For short-term use (up to 1 month), store at 4 °C.
• Keep the product in PBS formulation as supplied; do not dilute until ready for use.

General Application Notes

• Western Blot (WB): An initial dilution range of 1:500–1:2000 is recommended as a starting point; optimize empirically for your specific sample type and detection system.
• Immunofluorescence (IF) / Immunocytochemistry (ICC): Titrate antibody concentration according to cell type and fixation method used.
• Immunoprecipitation (IP): Use recommended amounts per standard IP protocols; confirm specificity with appropriate controls.

Controls & Validation

Always include a positive control (cell line or tissue known to express LYAR) and a negative control (isotype control mouse IgG) in each experiment to confirm specificity and rule out non-specific binding.

Troubleshooting Common Issues

If experimental results are unsatisfactory, consider the following troubleshooting steps:

• No signal detected: Verify that the target protein is expressed in your model system. Check antibody storage conditions and ensure it has not been subjected to repeated freeze-thaw cycles. Confirm secondary antibody compatibility with mouse primary antibody.
• High background: Increase blocking time or adjust blocking agent concentration. Reduce primary antibody concentration. Optimize wash steps to remove unbound antibody.
• Non-specific bands (WB): Increase antibody dilution, extend washing steps, or optimize blocking conditions. Confirm sample preparation integrity.
• Weak signal: Increase primary antibody concentration within the recommended range, extend incubation time, or optimize antigen retrieval conditions if applicable.

If issues persist, contact Aladdin Scientific technical support with lot number, experimental conditions, and results for expert assistance.

Ordering & Quality Assurance

LYAR Mouse mAb is available from Aladdin Scientific under SKU Ab350997. Each lot undergoes quality control testing to ensure consistent performance. A Certificate of Analysis (CoA) is available upon request and provides lot-specific concentration, purity, and validated application data.

For bulk orders, custom formulations, or additional technical documentation, please contact the Aladdin Scientific sales or technical support team. Standard shipping conditions for this antibody should maintain the cold chain; refer to the product datasheet for shipping and handling recommendations specific to this formulation.

Frequently asked questions

What is the recommended storage condition for LYAR Mouse mAb (Ab350997)?

For long-term storage, keep LYAR Mouse mAb at –20 °C. For short-term use up to one month, storage at 4 °C is acceptable. Avoid repeated freeze-thaw cycles, as these can compromise antibody integrity and performance.

In which applications can LYAR Mouse mAb be used?

LYAR Mouse mAb is intended for research applications. Typical applications for monoclonal antibodies of this type include Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), and Immunoprecipitation (IP). Researchers should consult the lot-specific Certificate of Analysis for confirmed validated applications for this product.

What formulation is LYAR Mouse mAb supplied in?

LYAR Mouse mAb (Ab350997) is supplied in PBS (phosphate-buffered saline) only. No additional stabilizers or preservatives are indicated in the source information. Please refer to the Certificate of Analysis for the exact formulation details of your specific lot.

How do I select appropriate controls when using LYAR Mouse mAb?

Include a positive control using a cell line or tissue known to express LYAR, and a negative control using an isotype-matched mouse IgG antibody. These controls help confirm antibody specificity and rule out non-specific binding in your experimental system.

What should I do if I observe high background when using LYAR Mouse mAb?

High background can often be reduced by increasing the blocking time or concentration of the blocking agent, reducing the primary antibody concentration, and optimizing wash steps to remove unbound antibody. If the issue persists, contact Aladdin Scientific technical support for further guidance.

References

1. Product information for LYAR Mouse mAb, Aladdin Scientific, SKU Ab350997 (absea#KC-29864). PBS formulation.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.