Laboratory Protocols & SOPs Library

Phosphate Buffered Saline (PBS) 10x

Recipe

1.Dissolve the following in 800ml distilled H2O.

  • 80g of NaCl

  • 2.0g of KCl

  • 14.4g of Na2HPO4

  • 2.4g of KH2PO4

2.Adjust pH to 7.4.

3.Adjust volume to 1L with additional distilled H2O.

4.Sterilize by autoclaving

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Phosphate Buffered Saline (PBS) 10x" Aladdin Knowledge Base, updated 9 juin 2025. https://www.aladdinsci.com/eu_fr/faqs/phosphate-buffered-saline-pbs-10x-en.html
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