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Phosphate Buffered Saline Tween-20 (PBST) 1x

Protocols

Phosphate Buffered Saline Tween-20 (PBST) 1x

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

1.Dissolve the following in 800 ml of distilled H2O

8g of NaCl
0.2g of KCl
1.44g of Na2HPO4
0.24g of KH2PO4
2ml of tween-20

2.Adjust pH to 7.2

3.Adjust volume to 1L with additional distilled H2O

4.Sterilize by autoclaving

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Phosphate Buffered Saline Tween-20 (PBST) 1x" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/phosphate-buffered-saline-tween-20-pbst-1x-en.html

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