Recombinant Human Anoctamin 1 Protein: Key FAQs

Recombinant Human Anoctamin 1 Protein is a recombinant form of the calcium-activated chloride channel ANO1 (also known as TMEM16A), produced for use in ion channel research, drug discovery, and functional studies requiring defined purity and activity.

Protein Name

Recombinant Human Anoctamin 1 Protein

Also Known As

ANO1, TMEM16A, DOG1, ORAOV2, TAOS2

Protein Function

Calcium-activated chloride channel involved in epithelial secretion, smooth muscle contraction, and nociception

SKU

rp369268

Supplier Reference

absea#PP-12375

Species

Human (Homo sapiens)

Content Type

Frequently Asked Questions (FAQ)

What Is Recombinant Human Anoctamin 1 Protein?

Recombinant Human Anoctamin 1 Protein, also designated ANO1 or TMEM16A, is a recombinant version of a transmembrane protein that functions as a calcium-activated chloride channel (CaCC). ANO1 is encoded by the ANO1 gene (also historically referenced as TMEM16A) and belongs to the anoctamin/TMEM16 family of proteins, which share a conserved eight-transmembrane topology.

ANO1 is widely expressed in epithelial tissues, smooth muscle, sensory neurons, and interstitial cells of Cajal. It plays essential roles in transepithelial chloride secretion, smooth muscle contractility, gastrointestinal motility, and pain signaling. In oncology research, ANO1 has attracted attention as a potential biomarker and therapeutic target, particularly in gastrointestinal stromal tumors (GISTs) and head-and-neck squamous cell carcinomas, where it is frequently amplified.

The recombinant protein supplied by Aladdin Scientific (SKU: rp369268) is intended for use in biochemical assays, binding studies, antibody validation, and other laboratory applications where a defined, research-grade ANO1 protein preparation is required.

Expression System, Purity, and Formulation

Recombinant proteins can be produced in a variety of host systems, each offering distinct advantages with respect to post-translational modifications, solubility, and yield. Common expression platforms for mammalian ion channel proteins such as ANO1 include E. coli, baculovirus-insect cell, and mammalian cell (HEK293 or CHO) systems.

For a structurally complex, multi-pass transmembrane protein like ANO1, mammalian or insect-cell expression systems are generally preferred because they support the correct folding, glycosylation, and membrane integration that may be required for functional and binding assays. Researchers should consult the product datasheet (absea#PP-12375 / SKU rp369268) for the confirmed expression host, tag information (e.g., His-tag, GST-tag), purity grade (typically ≥85% by SDS-PAGE for research-grade recombinant proteins), and formulation buffer details before designing experiments.

Note: Specific purity values, tag identity, and buffer composition for rp369268 are not stated in the current source material. Please refer to the official Aladdin Scientific product datasheet or contact technical support for confirmed specifications.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins is critical for maintaining activity and preventing degradation. General best practices for recombinant proteins applicable to ANO1 include:

• Short-term storage (≤1 week): Store at 4 °C in the supplied formulation buffer.
• Long-term storage: Aliquot the protein upon receipt and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can cause aggregation and loss of activity.
• Carrier protein: If the protein is supplied at low concentration, consider adding a carrier protein (e.g., 0.1% BSA) to improve stability during storage.
• Avoid repeated freeze-thaw: Each freeze-thaw cycle can incrementally reduce protein integrity.

Note: The confirmed storage conditions, shelf life, and reconstitution instructions specific to rp369268 are not included in the source material provided. Always refer to the product datasheet for authoritative storage guidance.

Recommended Applications and Research Context

Recombinant Human Anoctamin 1 Protein is suitable for a range of in vitro research applications. Typical uses for recombinant ANO1 protein preparations in the scientific literature include:

• Biochemical assays: ELISA, Western blot (as a positive control antigen), and dot blot for validating ANO1-specific antibodies.
• Binding and interaction studies: Pull-down assays or surface plasmon resonance (SPR) to characterize protein-protein or protein-ligand interactions involving ANO1.
• Drug screening: Use as a target protein in inhibitor or activator screening campaigns for the development of ANO1 modulators relevant to pain, secretory diarrhea, hypertension, or cancer.
• Structural studies: As a component in reconstitution experiments for cryo-EM or other structural biology workflows, subject to confirmed protein quality.

Researchers investigating gastrointestinal motility disorders, cystic fibrosis-related secretory defects, nociception, or ANO1-amplified cancers will find this recombinant protein particularly relevant. Always validate the protein's activity in your specific assay format using appropriate positive controls.

Ordering, Technical Support, and Quality Assurance

Recombinant Human Anoctamin 1 Protein is available from Aladdin Scientific under SKU rp369268 (supplier reference absea#PP-12375). To place an order or request a quote, contact your regional Aladdin Scientific representative or visit the Aladdin Scientific website.

Technical Support: For application-specific guidance, reconstitution protocols, or troubleshooting, Aladdin Scientific's technical support team is available to assist. Please have your SKU and lot number ready when contacting support to ensure the most accurate guidance.

Quality Assurance: Aladdin Scientific research-grade recombinant proteins undergo quality control testing prior to release. The specific QC tests performed on rp369268 (e.g., SDS-PAGE, activity assay, endotoxin testing) are detailed on the product datasheet. Researchers requiring low-endotoxin or GMP-grade material should enquire with the sales team about available options.

Returns and Replacements: If the product does not meet quality expectations, please contact Aladdin Scientific customer service in accordance with the company's returns and refunds policy.

Frequently asked questions

What is the biological function of Anoctamin 1 (ANO1/TMEM16A)?

ANO1 (TMEM16A) is a calcium-activated chloride channel (CaCC) that mediates chloride ion flux across the plasma membrane in response to elevated intracellular calcium. It plays critical roles in epithelial fluid secretion, smooth muscle contraction, gastrointestinal motility (via interstitial cells of Cajal), and pain signal transduction in sensory neurons. ANO1 is also overexpressed or amplified in several cancers, making it a subject of active oncological research.

Which expression system is used to produce rp369268, and does it affect protein quality?

The specific expression system used for rp369268 is not confirmed in the currently available source material; please consult the official product datasheet (absea#PP-12375) for this information. In general, for complex multi-pass transmembrane proteins like ANO1, mammalian or insect-cell (baculovirus) expression systems are preferred as they support proper protein folding and glycosylation, which can be critical for functional and binding studies.

How should I store Recombinant Human Anoctamin 1 Protein to maintain activity?

As a general best practice for recombinant proteins, aliquot upon receipt and store at −20 °C or −80 °C for long-term preservation, avoiding repeated freeze-thaw cycles. For short-term use within one week, storage at 4 °C in the supplied buffer is typically acceptable. Always refer to the specific storage instructions on the rp369268 product datasheet, as conditions may vary depending on formulation.

What applications is Recombinant Human Anoctamin 1 Protein (rp369268) validated for?

Recombinant ANO1 protein is broadly used in antibody validation (Western blot, ELISA positive controls), protein-protein interaction studies, drug target screening for ANO1 modulators, and as a reference protein in ion channel research. Confirmed validated applications for the specific lot rp369268 are listed on the product datasheet; researchers should verify suitability for their intended assay format prior to use.

Is Recombinant Human Anoctamin 1 Protein suitable for drug discovery targeting ANO1?

Yes, recombinant ANO1 protein is a relevant reagent for drug discovery programs targeting ANO1, including screening for small-molecule inhibitors or activators relevant to diseases such as secretory diarrhea, asthma, hypertension, pain, and ANO1-amplified cancers. Researchers should confirm protein activity and assay compatibility using the specifications on the rp369268 datasheet and appropriate functional controls.

How do I order Recombinant Human Anoctamin 1 Protein from Aladdin Scientific?

Recombinant Human Anoctamin 1 Protein can be ordered directly from Aladdin Scientific using SKU rp369268 via the Aladdin Scientific website or by contacting your regional sales representative. For bulk quantities, custom formulations, or pricing enquiries, please reach out to the Aladdin Scientific sales team directly.

References

1. Yang YD, et al. TMEM16A confers receptor-activated calcium-dependent chloride conductance. Nature. 2008;455(7217):1210-1215. doi:10.1038/nature07313
2. Caputo A, et al. TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity. Science. 2008;322(5901):590-594. doi:10.1126/science.1163518
3. Schroeder BC, et al. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell. 2008;134(6):1019-1029. doi:10.1016/j.cell.2008.09.003
4. Aladdin Scientific. Recombinant Human Anoctamin 1 Protein, SKU rp369268. Product Datasheet. absea#PP-12375.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.