Recombinant Human ATMIN Protein: FAQs & Key Facts

Recombinant Human ATMIN Protein is a research-grade recombinant protein representing the human ATM interactor (ATMIN), a nuclear factor involved in ATM kinase signaling, DNA damage response, and transcriptional regulation.

Product Name

Recombinant Human ATMIN Protein

SKU

rp376694

Protein Origin

Human (Homo sapiens)

Protein Class

Recombinant nuclear regulatory protein

Primary Research Area

DNA damage response, ATM kinase signaling, transcription regulation

Supplier Reference

absea#PP-10236

What Is ATMIN and Why Is It Relevant to Research?

ATMIN (ATM interactor, also known as ASCIZ or ZNF822) is a multifunctional nuclear protein encoded by the ATMIN gene in humans. It was originally identified as a binding partner and transcriptional target of ATM (Ataxia Telangiectasia Mutated) kinase, a central regulator of the DNA damage response (DDR) pathway.

ATMIN serves dual roles in the cell: it functions as a DNA damage response factor that facilitates ATM activation under specific genotoxic conditions, and as a transcription factor that regulates expression of dynein light chain proteins (e.g., DYNLL1/LC8). These dual roles make ATMIN a relevant target in studies of genome stability, cellular stress responses, and transcriptional networks.

Research applications for ATMIN include investigation of ATM-dependent signaling cascades, DNA repair pathway studies, transcription factor interaction assays, and protein-protein interaction experiments. Recombinant Human ATMIN Protein (rp376694) provides a defined, purified source of this protein to support such in vitro studies.

Product Specifications and Quality Information

Recombinant Human ATMIN Protein (SKU: rp376694) is produced as a recombinant protein derived from the human ATMIN sequence. The product is referenced under supplier code absea#PP-10236.

Key specification considerations for researchers:

• Species: Human (Homo sapiens)
• Format: Recombinant protein (specific tag, host expression system, and formulation details should be confirmed with the current product datasheet at time of ordering, as specifications may be updated)
• Purity: Recombinant proteins in this catalog are generally manufactured to research-grade purity; exact purity values should be verified on the product certificate of analysis (CoA)
• Application suitability: Typical applications include in vitro binding assays, ELISA standard preparation, Western blot positive controls, and functional biochemical assays — confirm specific validated applications on the datasheet

Researchers should always request the current CoA and Safety Data Sheet (SDS) prior to use to confirm lot-specific quality parameters.

Storage, Handling, and Stability Guidelines

Proper storage and handling of recombinant proteins is critical to maintaining biological activity and preventing degradation. While lot-specific conditions are always specified on the product datasheet and CoA, the following represent standard best practices for recombinant nuclear proteins of this type:

• Short-term storage (≤1 week): Store at 4°C in the supplied buffer
• Long-term storage: Aliquot and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can reduce activity
• Freeze-thaw cycles: Minimize to no more than 2–3 cycles; prepare single-use aliquots upon receipt
• Carrier protein addition: If working with dilute concentrations, consider adding a carrier protein (e.g., 0.1% BSA) to prevent adsorption to tube surfaces
• Avoid: Repeated freeze-thaw, prolonged exposure to room temperature, and detergent contamination

Always refer to the specific product datasheet for validated storage conditions for this SKU, as formulation details may vary by lot.

Recommended Applications and Experimental Considerations

Recombinant Human ATMIN Protein is suitable for a range of in vitro research applications. Researchers should validate the protein for each specific assay in their laboratory context. Common applications for recombinant ATMIN protein include:

• Protein-protein interaction studies: ATMIN interacts with ATM kinase and dynein light chain proteins; recombinant ATMIN can be used in pull-down assays or surface plasmon resonance (SPR) experiments to characterize these interactions
• Immunoassay standards: As a positive control or standard in ELISA-based quantification of ATMIN
• Western blot positive control: Confirm antibody specificity using purified recombinant protein
• In vitro functional assays: Investigate ATMIN's role in ATM activation or transcriptional regulation in cell-free systems
• Structural biology: Recombinant protein preparations can support biophysical characterization studies

Note: Specific validated applications are listed on the product datasheet. Researchers should perform optimization experiments, including titration and appropriate controls, before committing to large-scale studies.

Ordering, Documentation, and Support

Recombinant Human ATMIN Protein is available from Aladdin Scientific under SKU rp376694. The following guidance applies to ordering and post-purchase support:

• How to order: Place orders via the Aladdin Scientific website, by contacting your regional sales representative, or through approved procurement channels. Reference SKU rp376694 and supplier code absea#PP-10236 when ordering.
• Documentation available: Product datasheet, Safety Data Sheet (SDS), and Certificate of Analysis (CoA) are available upon request or via the product page. Always use the CoA specific to your received lot number.
• Technical support: For application-specific technical questions, contact Aladdin Scientific technical support. Our team can assist with recommended protocols, troubleshooting, and experimental design guidance.
• Returns and quality concerns: If you receive a product that does not meet specifications, contact customer service promptly with your order number and lot number for assistance in accordance with Aladdin's return and refund policy.

Frequently asked questions

What is the biological function of ATMIN?

ATMIN (ATM interactor) is a nuclear protein that functions both as a cofactor for ATM kinase in the DNA damage response and as a transcription factor regulating dynein light chain gene expression (e.g., DYNLL1). It plays roles in genome stability maintenance and cellular stress signaling.

What applications is Recombinant Human ATMIN Protein validated for?

Recombinant Human ATMIN Protein (rp376694) is suitable for applications including protein-protein interaction studies, Western blot positive controls, ELISA standards, and in vitro functional assays. Researchers should confirm specific validated applications on the current product datasheet, as validated use may vary by lot.

How should Recombinant Human ATMIN Protein be stored to maintain activity?

For short-term use (up to one week), store at 4°C. For long-term storage, aliquot the protein and store at −20°C or −80°C to avoid repeated freeze-thaw cycles. Minimize freeze-thaw cycles to 2–3 maximum and always consult the product datasheet for lot-specific storage instructions.

What is the SKU and supplier reference number for this product?

Recombinant Human ATMIN Protein is available from Aladdin Scientific under SKU rp376694. The supplier reference code is absea#PP-10236. Please use these identifiers when placing orders or contacting technical support.

Can Recombinant Human ATMIN Protein be used in cell-based assays?

Recombinant Human ATMIN Protein is primarily intended for in vitro biochemical and immunological assays. Use in cell-based assays would require additional validation including assessment of cell permeability or delivery methods. Researchers should consult the product datasheet and contact Aladdin Scientific technical support for guidance on specific experimental designs.

How do I request a Certificate of Analysis or Safety Data Sheet for this product?

The Certificate of Analysis (CoA) and Safety Data Sheet (SDS) for Recombinant Human ATMIN Protein (rp376694) are available through the product page on the Aladdin Scientific website or upon request from customer service. Please have your lot number ready when requesting a lot-specific CoA.

References

1. Stracker TH, Roig I, Knobel PA, Marjanovic M. The ATM signaling network in development and disease. Front Genet. 2013;4:37. doi:10.3389/fgene.2013.00037
2. Jurado S, Conlan LA, Baker EK, et al. ATM substrate Chk2-interacting Zn2+-finger (ASCIZ) Is a bi-functional transcriptional activator and feedback sensor in the regulation of dynein light chain (DYNLL1) expression. J Biol Chem. 2012;287(4):2579-2591. doi:10.1074/jbc.M111.306019
3. McNees CJ, Conlan LA, Tenis N, Heierhorst J. ASCIZ regulates lesion-specific Rad51 focus formation and apoptosis after methylating DNA damage. EMBO J. 2005;24(13):2447-2457. doi:10.1038/sj.emboj.7600704

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.