Recombinant Human C8orf34 Protein: Handling & Usage Protocol

Recombinant Human C8orf34 Protein is a research-grade recombinant protein intended for use in biochemical and cell-based studies, requiring careful reconstitution, aliquoting, and storage to maintain optimal activity and integrity.

Product Name

Recombinant Human C8orf34 Protein

SKU

rp375813

Species

Human (Homo sapiens)

Protein Type

Recombinant protein

Intended Use

Research use only (RUO)

Storage Recommendation

Avoid repeated freeze-thaw cycles; store aliquots at -80 °C for long-term use

Materials Required

Before beginning, ensure the following materials are available:

• Recombinant Human C8orf34 Protein (SKU: rp375813, Aladdin Scientific)
• Sterile, nuclease-free water or the recommended reconstitution buffer (refer to the product datasheet for specific buffer composition)
• Sterile low-binding microcentrifuge tubes (0.5 mL or 1.5 mL)
• Calibrated micropipettes and sterile tips
• Vortex mixer or tube rotator
• Refrigerated microcentrifuge
• Ice bucket or wet ice for sample handling on ice
• Appropriate PPE: lab coat, nitrile gloves, and safety glasses

Note: Always consult the product-specific datasheet (absea#PP-9462) supplied with the product for confirmed buffer compositions, concentrations, and any additional reagent requirements.

Reconstitution Procedure

Follow these steps to reconstitute Recombinant Human C8orf34 Protein:

1. Equilibrate the vial: Allow the lyophilized protein vial to equilibrate to room temperature (approximately 15–25 °C) for 10–15 minutes before opening to minimize condensation and protein aggregation.
2. Brief centrifugation: Centrifuge the sealed vial briefly (e.g., 3,000 × g for 30 seconds) to collect any lyophilized material from the sides and cap of the vial.
3. Add reconstitution buffer: Add the volume of sterile reconstitution buffer specified in the product datasheet (absea#PP-9462) slowly and gently to the side of the vial wall rather than directly onto the lyophilized cake to avoid foaming.
4. Gentle mixing: Do not vortex vigorously. Gently swirl or rotate the vial for 5–10 minutes at room temperature until the protein is fully dissolved. If necessary, allow the vial to stand at 4 °C for an additional 15 minutes with occasional gentle agitation.
5. Clarification: Briefly centrifuge the reconstituted solution (e.g., 10,000 × g for 1 minute at 4 °C) to remove any insoluble particulates.
6. Verify dissolution: Visually inspect the solution; it should appear clear. If visible aggregates remain, repeat gentle agitation before proceeding.

Important: Confirm the recommended reconstitution volume and buffer from the product datasheet prior to use, as specific conditions may vary by lot.

Storage and Stability

Proper storage is critical to maintaining the activity and stability of Recombinant Human C8orf34 Protein:

• Short-term storage: If the protein will be used within 1–2 weeks, store the reconstituted solution at 4 °C. Adding a carrier protein such as 0.1% BSA (bovine serum albumin) may help stabilize the protein during short-term storage; consult the datasheet for specific recommendations.
• Long-term storage: For storage beyond two weeks, prepare single-use aliquots in low-binding microcentrifuge tubes and store at -80 °C. Cryoprotectants such as glycerol (typically 5–10% final concentration) may be added if recommended by the datasheet.
• Avoid freeze-thaw cycles: Repeated freeze-thaw cycles significantly degrade protein integrity and reduce biological activity. Use single-use aliquots to prevent this.
• Do not store at -20 °C for extended periods unless the datasheet specifically recommends this temperature.
• Expiry: Always observe the expiry date printed on the vial label and document storage conditions in your laboratory notebook.

Note: Stability data specific to this product should be confirmed with the product datasheet (absea#PP-9462) or by contacting Aladdin Scientific technical support.

Experimental Application Guidelines

Recombinant Human C8orf34 Protein can be used in a range of biochemical and cell-based research applications. General guidelines for use are provided below; researchers should optimize conditions for their specific experimental systems:

• Protein–protein interaction studies: C8orf34 protein may be used as bait or prey in pull-down or co-immunoprecipitation assays to identify interacting partners. Ensure appropriate controls (e.g., negative control with unrelated recombinant protein) are included.
• SDS-PAGE and Western blot validation: Prior to use in downstream assays, confirm protein integrity and purity by running an aliquot on SDS-PAGE under reducing and non-reducing conditions, and verify by Western blot using an anti-C8orf34 antibody if available.
• Functional assays: Depending on the known or putative function of C8orf34 (chromosome 8 open reading frame 34), design cell-based or biochemical assays accordingly. Consult current literature for the most up-to-date functional data on this protein.
• Working concentration: The optimal working concentration must be determined empirically for each assay type. Prepare a dilution series and perform a dose-response experiment as a starting approach.

Disclaimer: This product is for research use only and is not intended for diagnostic, therapeutic, or clinical applications.

Troubleshooting

If you encounter difficulties with Recombinant Human C8orf34 Protein, refer to the following troubleshooting guide:

Problem	Possible Cause	Recommended Action
Protein does not dissolve completely	Insufficient reconstitution buffer volume; protein aggregation	Add a small additional volume of buffer; incubate at 4 °C with gentle rotation for 30 minutes; centrifuge and use the supernatant
Loss of activity after storage	Freeze-thaw degradation; improper storage temperature	Ensure single-use aliquots stored at -80 °C; check that storage conditions have been consistently maintained
Unexpected band size on SDS-PAGE	Post-translational modifications; protein tags; degradation	Verify under both reducing and non-reducing conditions; compare with the expected MW on the datasheet; check sample preparation
Low signal in functional assay	Sub-optimal protein concentration; assay conditions not optimized	Perform concentration optimization; review buffer composition and assay conditions; consult Aladdin Scientific technical support

For additional technical assistance, contact Aladdin Scientific customer and technical support, referencing product SKU rp375813.

Frequently asked questions

What buffer should I use to reconstitute Recombinant Human C8orf34 Protein?

The recommended reconstitution buffer and volume are specified in the product datasheet (absea#PP-9462) supplied with SKU rp375813. Typically, sterile water or a PBS-based buffer is used; however, always confirm with the specific datasheet before proceeding to ensure optimal protein solubility and stability.

How should Recombinant Human C8orf34 Protein be stored after reconstitution?

After reconstitution, prepare single-use aliquots in low-binding microcentrifuge tubes and store them at -80 °C for long-term stability. For use within 1–2 weeks, storage at 4 °C is acceptable. Avoid repeated freeze-thaw cycles, as these can significantly reduce protein integrity and activity.

Is Recombinant Human C8orf34 Protein suitable for use in cell-based assays?

Recombinant Human C8orf34 Protein is intended for research use and may be applicable in cell-based assays depending on the experimental objective. Researchers should optimize the working concentration and assay conditions empirically. This product is for research use only and is not approved for diagnostic or therapeutic applications.

How do I verify the purity and integrity of Recombinant Human C8orf34 Protein?

Purity and integrity can be assessed by running an aliquot on SDS-PAGE under both reducing and non-reducing conditions and comparing the observed band size to the expected molecular weight stated in the product datasheet. Western blotting with an anti-C8orf34 antibody provides additional confirmation of protein identity.

What is C8orf34, and what is its biological relevance?

C8orf34 (Chromosome 8 Open Reading Frame 34) is a human protein encoded on chromosome 8. The precise biological function of C8orf34 is still under investigation; researchers are encouraged to consult the current literature for the latest findings on its role in cellular processes and disease associations.

References

1. Aladdin Scientific. Recombinant Human C8orf34 Protein, SKU rp375813. Product Datasheet absea#PP-9462.
2. Wingfield PT. Overview of the purification of recombinant proteins. Current Protocols in Protein Science. 2015;80:6.1.1-6.1.35.
3. Structural Genomics Consortium and various contributing authors. Recombinant protein expression and purification: best practices. [General reference — specific citation to be confirmed].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.