Recombinant Human FAM184B Protein: Key FAQs

Recombinant Human FAM184B Protein is a research-grade recombinant protein derived from the human FAM184B gene, designed to support biochemical, cell biology, and functional studies requiring a well-characterized human protein reagent.

Product Name

Recombinant Human FAM184B Protein

SKU

rp375392

Gene Name

FAM184B (Family with Sequence Similarity 184, Member B)

Species Origin

Human (Homo sapiens)

Format

Recombinant protein; exact formulation details should be confirmed on the product datasheet

Intended Use

Research use only (RUO); not for diagnostic or therapeutic use

What Is FAM184B and Why Is It Studied?

FAM184B (Family with Sequence Similarity 184, Member B) is a human protein-coding gene whose precise biological function remains an active area of investigation. As a member of the FAM184 family, FAM184B has been identified in genome-wide association studies (GWAS) as a locus of interest in several biological contexts, including musculoskeletal and metabolic traits. Researchers study FAM184B to better understand its molecular interactions, potential regulatory roles, and relevance to human physiology and disease.

Recombinant Human FAM184B Protein provides a purified, defined source of this protein for in vitro experiments, enabling researchers to conduct binding assays, antibody validation, structural studies, and functional characterization without relying on endogenous protein extraction from human tissue.

Product Specifications and Quality

Recombinant Human FAM184B Protein (SKU: rp375392) is produced as a recombinant protein intended for research use. Key quality attributes for recombinant proteins typically include:

• Purity: Recombinant proteins from Aladdin Scientific are generally produced to high purity standards, typically assessed by SDS-PAGE and/or HPLC. Specific purity data for FAM184B should be confirmed on the product datasheet.
• Expression System: The expression host (e.g., E. coli, mammalian, insect, or yeast cells) determines post-translational modification status. Please refer to the official product page or datasheet for the confirmed expression system used for this specific product.
• Endotoxin Level: For functional cell-based assays, endotoxin content is a critical quality parameter. Consult the product datasheet for endotoxin specifications.
• Formulation: The buffer composition and any stabilizing additives are detailed on the product datasheet. Researchers should verify formulation compatibility with their assay systems prior to use.

For the most current and authoritative specifications, always refer to the official Aladdin Scientific product page for SKU rp375392.

Storage, Handling, and Reconstitution

Proper storage and handling are essential to preserve protein activity and integrity. The following general guidelines apply to recombinant proteins; always defer to the specific instructions provided on the FAM184B product datasheet:

• Storage Temperature: Recombinant proteins are typically stored at -20°C or -80°C upon receipt to maintain stability. Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Aliquoting: Upon first thaw, it is recommended to prepare single-use aliquots to minimize freeze-thaw stress on the protein.
• Reconstitution: If supplied in lyophilized form, reconstitute with the buffer specified in the datasheet. Allow the vial to equilibrate to room temperature before opening, and centrifuge briefly before reconstitution to collect material at the bottom of the vial.
• Working Concentration: Determine the optimal working concentration empirically for each assay type. Standard titration experiments are recommended.

Contact Aladdin Scientific technical support if you have questions about storage conditions specific to this product.

Recommended Applications and Usage Tips

Recombinant Human FAM184B Protein is suitable for a range of in vitro research applications. Common uses for recombinant proteins of this type include:

• Antibody Production and Validation: Use as an immunogen for antibody generation or as a positive control antigen in Western blot, ELISA, and immunoprecipitation experiments.
• Protein–Protein Interaction Studies: Employ in pull-down assays, co-immunoprecipitation setups, or surface plasmon resonance (SPR) experiments to identify binding partners.
• Structural Biology: Can be used as a substrate for crystallography or other structural characterization methods, subject to sufficient purity and homogeneity.
• Functional Assays: Depending on the biological role of FAM184B established in your model system, the recombinant protein may be used in cell-free or cell-based functional assays.

Users are advised to optimize assay conditions, including protein concentration, buffer composition, incubation time, and temperature, for their specific experimental needs. Aladdin Scientific's technical team is available to provide application-specific guidance.

Ordering, Shipping, and Returns

Recombinant Human FAM184B Protein (SKU: rp375392) can be ordered directly through the Aladdin Scientific website or by contacting your regional sales representative. Key logistical considerations include:

• Shipping Conditions: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity during transit. Confirm shipping conditions with Aladdin Scientific at time of order.
• Lead Time: Lead times may vary depending on stock availability. Check the product page for current availability or contact customer service for an estimated delivery timeline.
• Returns and Refunds: If you receive a product that does not meet specifications, contact Aladdin Scientific customer service promptly. Returns are subject to the company's standard returns and refunds policy. Documentation of the quality issue (e.g., photographs, data) will facilitate the process.
• Bulk and Custom Orders: For large-quantity requirements or custom formulations, please contact Aladdin Scientific directly to discuss options and pricing.

Frequently asked questions

What is the biological function of FAM184B?

FAM184B (Family with Sequence Similarity 184, Member B) is a protein-coding gene whose precise molecular function is still being characterized. It has been implicated in GWAS studies associated with certain musculoskeletal and metabolic traits. Researchers use the recombinant protein to investigate its interactions, expression patterns, and potential functional roles in human biology.

What expression system is used to produce Recombinant Human FAM184B Protein?

The specific expression system used for Recombinant Human FAM184B Protein (SKU: rp375392) should be confirmed by consulting the official product datasheet available on the Aladdin Scientific website. Common expression systems for recombinant human proteins include E. coli, mammalian (HEK293 or CHO), baculovirus/insect cell, and yeast systems, each affecting post-translational modifications and protein folding.

How should Recombinant Human FAM184B Protein be stored to maintain activity?

Recombinant Human FAM184B Protein should be stored according to the conditions specified on its product datasheet. In general, recombinant proteins are stored at -20°C or -80°C and should be aliquoted upon first thaw to avoid repeated freeze-thaw cycles. Always refer to the specific storage instructions provided with the product for optimal stability.

What applications is Recombinant Human FAM184B Protein validated for?

Recombinant Human FAM184B Protein is suitable for research applications including antibody production and validation, protein–protein interaction assays, ELISA, and structural studies. Validated applications and recommended concentrations are detailed on the product datasheet. Users should optimize conditions for their specific assay system.

Is Recombinant Human FAM184B Protein suitable for in vivo or clinical use?

No. Recombinant Human FAM184B Protein is intended for research use only (RUO) and is not approved or validated for diagnostic, therapeutic, or clinical applications. It must not be administered to humans or animals.

References

1. Product page: Recombinant Human FAM184B Protein, SKU rp375392, Aladdin Scientific. Refer to the official datasheet for full specifications.
2. NCBI Gene Entry: FAM184B, Homo sapiens. National Center for Biotechnology Information.
3. UniProt entry for FAM184B human protein. UniProt Consortium.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.