Recombinant Human GLCCI1 Protein: Key Facts & FAQs

Recombinant Human GLCCI1 Protein is a research-grade recombinant protein derived from the human GLCCI1 gene, designed to support studies in glucocorticoid-induced signaling, autophagy regulation, and related biomedical research applications.

Product Name

Recombinant Human GLCCI1 Protein

SKU

rp372296

Gene Name

GLCCI1 (Glucocorticoid-Induced Cancer Candidate 1)

Species

Human (Homo sapiens)

Application

In vitro research; functional assays; biochemical studies

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles

Supplier Reference

absea#PP-6345

What Is GLCCI1 and Why Is It Important in Research?

GLCCI1 (Glucocorticoid-Induced Cancer Candidate 1) is a protein encoded by the GLCCI1 gene in humans. It was originally identified as a gene whose expression is upregulated in response to glucocorticoid stimulation. Research has implicated GLCCI1 in several biological processes, including autophagy regulation and intracellular vesicle trafficking.

Studies have suggested that GLCCI1 plays a role in modulating glucocorticoid sensitivity, making it of particular interest in the context of asthma treatment response, inflammatory diseases, and cancer biology. Its involvement in autophagic pathways also positions it as a relevant target for research into cellular stress responses and disease mechanisms.

Recombinant Human GLCCI1 Protein (rp372296) enables researchers to investigate the biochemical functions of GLCCI1 under controlled in vitro conditions, providing a defined, reproducible reagent for mechanistic studies.

Product Specifications and Quality Attributes

Recombinant Human GLCCI1 Protein (SKU: rp372296) is produced to research-grade standards. Key specifications to consider when evaluating this product include:

• Source: Recombinant expression of the human GLCCI1 protein sequence.
• Purity: Manufactured to a purity level suitable for in vitro biochemical and functional assays; specific purity data should be confirmed in the product datasheet.
• Formulation: Supplied in a buffer compatible with standard biochemical applications; refer to the product datasheet for exact formulation details.
• Molecular Weight: Consistent with the predicted molecular weight of human GLCCI1; verify against the certificate of analysis.
• Supplier Reference: This product is referenced under absea#PP-6345 from the upstream supplier.

Researchers are strongly advised to consult the product datasheet and certificate of analysis (CoA) provided with each lot for exact specifications, including purity percentage, endotoxin levels, and activity data.

Recommended Applications and Experimental Use

Recombinant Human GLCCI1 Protein is intended for in vitro research use only and is not approved for diagnostic or therapeutic applications. Typical research applications for recombinant proteins of this class include:

• Protein–protein interaction studies: Investigating binding partners of GLCCI1 in autophagy or glucocorticoid signaling pathways.
• Biochemical assays: Use as a standard or positive control in ELISA, Western blot, or activity-based assays where available.
• Structural studies: Supporting crystallography or biophysical characterization of the GLCCI1 protein.
• Functional assays: Assessing the role of GLCCI1 in modulating cellular responses in cell-free or reconstituted systems.

Researchers should optimize experimental conditions, including protein concentration, buffer composition, and incubation parameters, according to their specific assay requirements. Pilot experiments are recommended to establish optimal working concentrations.

Storage, Handling, and Stability Guidelines

Proper storage and handling of Recombinant Human GLCCI1 Protein are critical to maintaining protein integrity and experimental reproducibility. The following general best practices apply:

• Long-term storage: Store at -80°C for extended periods to maximize protein stability and shelf life.
• Short-term storage: If the protein will be used within days to weeks, storage at -20°C is generally acceptable.
• Freeze-thaw cycles: Repeated freeze-thaw cycles should be avoided, as they can lead to protein aggregation and loss of activity. Aliquot the protein into single-use volumes upon receipt.
• Working solutions: Prepare working dilutions immediately before use in a compatible buffer. Keep on ice during experiments.
• Contamination prevention: Use aseptic technique and sterile consumables when handling the protein to prevent microbial contamination.

Always refer to the product-specific datasheet for the exact recommended storage conditions and stability data validated for this lot.

Ordering, Technical Support, and Quality Assurance

Recombinant Human GLCCI1 Protein is available from Aladdin Scientific under SKU rp372296. The following information is intended to assist researchers and procurement teams:

• Ordering: The product can be ordered directly through the Aladdin Scientific website or by contacting our sales team. Please quote SKU rp372296 to ensure accurate order processing.
• Documentation: A certificate of analysis (CoA) and safety data sheet (SDS) are available for each product lot. Contact customer service or access them via the product page on our website.
• Technical Support: Our scientific support team can assist with application recommendations, experimental design guidance, and troubleshooting. Reach out through the contact details on the Aladdin Scientific website.
• Quality Assurance: All Aladdin Scientific recombinant proteins are subject to quality control testing prior to release. Results are documented in the lot-specific CoA.
• Returns and Replacements: In the event of product quality concerns, please contact our customer service team. Returns and replacements are handled in accordance with Aladdin Scientific's standard returns policy.

Frequently asked questions

What is GLCCI1 and what biological function does it serve?

GLCCI1, or Glucocorticoid-Induced Cancer Candidate 1, is a human protein whose expression is upregulated by glucocorticoid stimulation. It has been implicated in autophagy regulation and intracellular vesicle trafficking, and research suggests it may influence glucocorticoid sensitivity in conditions such as asthma and inflammatory diseases.

What are the recommended storage conditions for Recombinant Human GLCCI1 Protein?

For long-term storage, keep the protein at -80°C. Short-term storage at -20°C is acceptable for use within days to weeks. Avoid repeated freeze-thaw cycles by aliquoting the protein into single-use volumes upon receipt, and always consult the product datasheet for lot-specific storage recommendations.

What research applications is Recombinant Human GLCCI1 Protein suitable for?

This protein is designed for in vitro research use and is suitable for applications such as protein–protein interaction studies, Western blot, ELISA, functional assays, and structural biology studies. It is not intended for diagnostic or therapeutic use. Researchers should optimize working concentrations for their specific experimental systems.

How do I obtain a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for each lot of Recombinant Human GLCCI1 Protein (rp372296). You can access it through the product page on the Aladdin Scientific website or by contacting our customer service team and providing your lot number.

Is Recombinant Human GLCCI1 Protein available for use in clinical or diagnostic settings?

No. Recombinant Human GLCCI1 Protein (rp372296) is for research use only (RUO) and is not approved, validated, or intended for clinical diagnosis, therapeutic applications, or use in humans or animals in a clinical context.

References

1. Product reference: absea#PP-6345. Supplier documentation for Recombinant Human GLCCI1 Protein. Aladdin Scientific SKU rp372296.
2. Tantisira KG et al. GLCCI1 and glucocorticoid response in asthma. General literature reference — specific citation details require verification.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.