Recombinant Human GOLGA8O Protein: FAQs & Key Facts

Recombinant Human GOLGA8O Protein is a research-grade recombinant protein derived from the human GOLGA8O gene, associated with Golgi apparatus structure and function, and is available from Aladdin Scientific (SKU rp377470) for use in a range of biochemical and cell biology research applications.

Product Name

Recombinant Human GOLGA8O Protein

SKU

rp377470

Gene / Protein Family

GOLGA8O; member of the Golgin family associated with Golgi apparatus organization

Species Origin

Human (Homo sapiens)

Recommended Storage

Store at −20 °C or −80 °C; avoid repeated freeze-thaw cycles

Supplier

Aladdin Scientific

What Is Recombinant Human GOLGA8O Protein?

Recombinant Human GOLGA8O Protein (SKU rp377470) is a recombinant form of the human GOLGA8O protein, a member of the Golgin protein family. Golgins are coiled-coil proteins predominantly localized to the Golgi apparatus, where they play roles in maintaining Golgi structure, vesicular trafficking, and membrane tethering. GOLGA8O represents a paralog within the GOLGA8 subfamily, which has expanded in the human genome relative to other primates.

As a recombinant protein produced under controlled laboratory conditions, this product is intended for research use only and provides a reproducible, well-defined protein source for downstream biochemical assays and cell biology studies.

Recommended Research Applications

Recombinant Human GOLGA8O Protein is suitable for a variety of research applications, including but not limited to:

• In vitro binding assays: Investigating protein–protein interactions involving Golgi-associated machinery.
• Antibody production and validation: Serving as an immunogen or positive control antigen for antibody development and specificity testing.
• Functional studies: Exploring the role of GOLGA8O in Golgi apparatus integrity and vesicular trafficking pathways.
• SDS-PAGE and Western blot standards: Use as a molecular weight reference or positive control.

Researchers should validate the suitability of this protein for their specific experimental systems and conditions prior to large-scale use.

Storage, Handling, and Stability

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. The following general guidelines apply to Recombinant Human GOLGA8O Protein:

• Temperature: Store at −20 °C for short-term use or −80 °C for long-term storage.
• Freeze-thaw cycles: Minimize repeated freeze-thaw cycles, as these can lead to protein aggregation and loss of activity. Aliquot the protein upon first thaw to avoid repeated cycling.
• Working conditions: Keep on ice during experimental workflows. Avoid prolonged exposure to room temperature.
• Reconstitution: If supplied lyophilized, reconstitute according to the product datasheet instructions using the recommended buffer.

Specific storage conditions, formulation details, and shelf-life data should be confirmed on the official product datasheet available from Aladdin Scientific.

Ordering and Technical Support

Recombinant Human GOLGA8O Protein can be ordered directly from Aladdin Scientific under SKU rp377470. The following information is useful for procurement and research teams:

• Quote and bulk inquiries: Contact Aladdin Scientific's sales team for volume pricing, custom formulations, or bulk order requests.
• Technical questions: Aladdin Scientific's technical support team can assist with application suitability, reconstitution protocols, and troubleshooting.
• Documentation: Safety Data Sheets (SDS) and product datasheets are available upon request or through the Aladdin Scientific website.
• Returns and refunds: Refer to Aladdin Scientific's standard returns and refunds policy for recombinant proteins; ensure cold-chain handling requirements are met during shipping.

Frequently asked questions

What is the function of GOLGA8O and why is it relevant to research?

GOLGA8O is a member of the Golgin protein family, which are coiled-coil proteins associated with the Golgi apparatus. Golgins are involved in maintaining Golgi structure, membrane tethering, and vesicular trafficking. GOLGA8O belongs to the GOLGA8 subfamily, which has undergone copy-number expansion in the human lineage, making it of interest in studies of human-specific genomic evolution and Golgi biology.

How should I store Recombinant Human GOLGA8O Protein to maintain its stability?

Store Recombinant Human GOLGA8O Protein at −20 °C for short-term storage or −80 °C for long-term preservation. To prevent activity loss due to protein aggregation, aliquot the protein into single-use volumes upon first thaw and avoid repeated freeze-thaw cycles. Always keep the protein on ice during experimental procedures.

What applications is Recombinant Human GOLGA8O Protein validated for?

Recombinant Human GOLGA8O Protein is generally suitable for in vitro binding assays, antibody production and validation, functional studies of Golgi-associated pathways, and use as a Western blot or SDS-PAGE standard. Researchers are advised to consult the official product datasheet for application-specific validation data and to perform their own optimization for their experimental systems.

How do I place an order for Recombinant Human GOLGA8O Protein from Aladdin Scientific?

You can order Recombinant Human GOLGA8O Protein using SKU rp377470 directly through the Aladdin Scientific website or by contacting the sales team for assistance with bulk orders, custom requirements, or pricing inquiries. Technical documentation including the SDS and product datasheet are available upon request.

Can Recombinant Human GOLGA8O Protein be used in cell-based assays?

Recombinant Human GOLGA8O Protein may be applicable to certain cell-based assays; however, suitability depends on the specific experimental design, delivery method, and cell type. Researchers should consult the product datasheet and conduct pilot experiments to determine compatibility with their cell-based system before proceeding with larger studies.

References

1. Aladdin Scientific. Recombinant Human GOLGA8O Protein, SKU rp377470. Product datasheet.
2. Barr FA. Review series: Rab GTPases and membrane identity: causal or inconsequential? J Cell Biol. 2013;202(2):191-199. [General Golgi/Golgin biology reference — verify applicability to GOLGA8O specifically]
3. Golgins and Golgi structure — general textbook-level reference on coiled-coil tethering factors at the Golgi apparatus. [Specific citation to be added after literature review]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.