Recombinant Human GREB1 Protein: Properties & Research Uses

Recombinant Human GREB1 Protein is a research-grade recombinant protein that enables scientists to investigate the molecular mechanisms of estrogen receptor signaling, gene regulation, and hormone-dependent oncogenesis in vitro.

Product Name

Recombinant Human GREB1 Protein

SKU

rp366671

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Primary Research Area

Estrogen receptor signaling, hormone-dependent cancers, chromatin regulation

Supplier

Aladdin Scientific

What Is GREB1 and Why Does It Matter?

GREB1 (Growth Regulation by Estrogen in Breast Cancer 1) is a protein-coding gene originally identified as an early estrogen-responsive gene in breast cancer cell lines. The GREB1 protein functions as a coregulator within the estrogen receptor (ER) transcriptional complex, contributing to the activation of estrogen-responsive genes. It plays a central role in mediating estrogen-dependent proliferation of breast and other hormone-sensitive cancer cells.

Beyond breast cancer, GREB1 has been implicated in prostate cancer, ovarian cancer, and endometriosis, making it a subject of broad oncological and reproductive biology research. Understanding GREB1 protein interactions and regulatory functions requires high-quality recombinant protein tools that faithfully replicate the native protein's biochemical properties.

Biochemical Properties of Recombinant Human GREB1 Protein

Recombinant Human GREB1 Protein (rp366671) is produced to support demanding in vitro research applications. As a recombinant protein derived from the human GREB1 gene sequence, it provides a well-defined reagent for biochemical assays, protein–protein interaction studies, and structural investigations.

Key biochemical considerations for GREB1 include its large molecular weight, as the full-length human GREB1 protein is encoded by a transcript spanning multiple exons and producing a high-molecular-weight product. Researchers should verify purity, formulation buffer, storage conditions, and recommended handling procedures in the accompanying product datasheet (absea#PP-2238) to ensure experimental reproducibility.

The recombinant format ensures batch-to-batch consistency that is difficult to achieve with endogenous protein preparations, making rp366671 suitable for quantitative biochemical experiments.

Research Applications

Recombinant Human GREB1 Protein supports a variety of research applications in cancer biology, endocrinology, and molecular biology:

• Estrogen Receptor Signaling Studies: GREB1 is a direct transcriptional target and coregulator of ERα. Recombinant GREB1 protein can be used to dissect ER–GREB1 interaction interfaces and map functional domains.
• Protein–Protein Interaction Assays: Pull-down assays, co-immunoprecipitation controls, and surface plasmon resonance (SPR) experiments can employ rp366671 to identify novel GREB1-interacting partners.
• Biochemical and Enzymatic Assays: The recombinant protein serves as a standard or positive control in Western blot, ELISA, and dot blot assays where GREB1 detection and quantification are required.
• Drug Discovery and Target Validation: GREB1's role in hormone-dependent cancers makes it a relevant target for screening small-molecule inhibitors of ER coactivator interactions.
• Structural Biology: Purified recombinant GREB1 fragments or full-length protein can support crystallography or cryo-EM studies aimed at resolving GREB1 domain architecture.

Storage, Handling, and Quality Considerations

Proper storage and handling of Recombinant Human GREB1 Protein (rp366671) are essential to maintain protein integrity and biological activity. Researchers should consult the official product datasheet (absea#PP-2238) for specific guidance on:

• Storage temperature: Recombinant proteins are typically stored at −20 °C or −80 °C; avoid repeated freeze–thaw cycles.
• Reconstitution: If supplied lyophilized, reconstitute in the recommended buffer as specified in the datasheet and allow complete solubilization before use.
• Aliquoting: Prepare single-use aliquots upon receipt to minimize freeze–thaw degradation.
• Protein stability: Carrier proteins such as BSA may be included in the formulation to enhance stability; verify formulation details in the datasheet.

All specific purity percentages, concentration values, and formulation details should be confirmed against the current product datasheet, as specifications may be updated between manufacturing lots.

Ordering Information

Recombinant Human GREB1 Protein is available from Aladdin Scientific under SKU rp366671. This product is intended for research use only and is not approved for diagnostic or therapeutic applications.

For pricing, available quantities, and current stock status, please contact Aladdin Scientific customer service or visit the product page. Custom formulations, larger quantities, or bulk orders may be available upon request. Refer to product datasheet absea#PP-2238 for full technical specifications prior to placing an order.

Frequently asked questions

What is GREB1 protein and what is its biological function?

GREB1 (Growth Regulation by Estrogen in Breast Cancer 1) is an estrogen-responsive protein that acts as a coregulator within the estrogen receptor transcriptional complex. It promotes estrogen-dependent gene expression and cell proliferation, and has been implicated in hormone-driven cancers including breast, prostate, and ovarian cancer, as well as endometriosis.

What research applications is Recombinant Human GREB1 Protein (rp366671) suitable for?

Recombinant Human GREB1 Protein is suitable for protein–protein interaction assays (e.g., pull-down, co-IP), Western blot and ELISA as a positive control or standard, drug discovery screening for ER coactivator inhibitors, and structural biology studies. Always verify suitability for specific assay formats against the product datasheet.

How should Recombinant Human GREB1 Protein be stored to maintain activity?

Recombinant proteins including GREB1 should generally be stored at −20 °C or −80 °C and should not be subjected to repeated freeze–thaw cycles. It is recommended to prepare single-use aliquots upon receipt. Please consult the official product datasheet (absea#PP-2238) for the exact storage conditions and reconstitution instructions specific to this product.

Is Recombinant Human GREB1 Protein (rp366671) suitable for clinical or diagnostic use?

No. Recombinant Human GREB1 Protein (rp366671) is intended for research use only. It has not been validated or approved for use in clinical diagnostics, therapeutic applications, or human/veterinary medical procedures.

References

1. Mohammed H, et al. Progesterone receptor modulates ERα action in breast cancer. Nature. 2015.
2. Rae JM, et al. GREB1 is a critical regulator of hormone dependent breast cancer growth. Breast Cancer Research and Treatment. 2005.
3. Absea Biotechnology. Product datasheet PP-2238: Recombinant Human GREB1 Protein. [Internal reference].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.